A gold glyco-nanoparticle carrying a Listeriolysin O peptide and formulated with Advax™ delta inulin adjuvant induces robust T-cell protection against listeria infection
In the search for an effective vaccine against the human pathogen, Listeria monocytogenes (Listeria), gold glyconanoparticles (GNP) loaded with a listeriolysin O peptide LLO91-99 (GNP-LLO) were used to immunise mice, initially using a dendritic cell (DC) vaccine approach, but subsequently using a standard parenteral immunisation approach. To enhance vaccine immunogenicity a novel polysaccharide adjuvant based on delta inulin (Advax™) was also co-formulated with the GNP vaccine. Confirming previous results, DC loaded in vitro with GNP-LLO provided better protection against listeriosis than DC loaded in vitro using free LLO peptide. The immunogenicity of GNP-LLO loaded DC vaccines was further increased by addition of Advax™ adjuvant. However, as DC vaccines are expensive and impracticable for prophylactic use, we next asked whether the same GNP-LLO antigen could be used to directly target DC in vivo. Immunisation of mice with GNP-LLO plus Advax™ adjuvant induced LLO-specific T-cell immunity and protection against Listeria challenge. Protection correlated with an increased frequency of splenic CD4(+) and CD8(+) T cells, NK cells and CD8α(+) DC, and Th1 cytokine production (IL-12, IFN-γ, TNF-α, and MCP-1), post-challenge. Enhanced T-cell epitope recruitment post-challenge was seen in the groups that received Advax™ adjuvant. Immunisation with GNP-LLO91-99 plus Advax™ adjuvant provided equally robust Listeria protection as the best DC vaccine strategy but without the complexity and cost, making this a highly promising strategy for development of a prophylactic vaccine against listeriosis..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Vaccine - 33(2015), 12, Seite 1465-1473 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rodriguez-Del Rio, Estela [VerfasserIn] |
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| Links: |
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| doi: |
10.1016/j.vaccine.2015.01.062 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Rodriguez-Del Rio, Estela |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A gold glyco-nanoparticle carrying a Listeriolysin O peptide and formulated with Advax™ delta inulin adjuvant induces robust T-cell protection against listeria infection |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a In the search for an effective vaccine against the human pathogen, Listeria monocytogenes (Listeria), gold glyconanoparticles (GNP) loaded with a listeriolysin O peptide LLO91-99 (GNP-LLO) were used to immunise mice, initially using a dendritic cell (DC) vaccine approach, but subsequently using a standard parenteral immunisation approach. To enhance vaccine immunogenicity a novel polysaccharide adjuvant based on delta inulin (Advax™) was also co-formulated with the GNP vaccine. Confirming previous results, DC loaded in vitro with GNP-LLO provided better protection against listeriosis than DC loaded in vitro using free LLO peptide. The immunogenicity of GNP-LLO loaded DC vaccines was further increased by addition of Advax™ adjuvant. However, as DC vaccines are expensive and impracticable for prophylactic use, we next asked whether the same GNP-LLO antigen could be used to directly target DC in vivo. Immunisation of mice with GNP-LLO plus Advax™ adjuvant induced LLO-specific T-cell immunity and protection against Listeria challenge. Protection correlated with an increased frequency of splenic CD4(+) and CD8(+) T cells, NK cells and CD8α(+) DC, and Th1 cytokine production (IL-12, IFN-γ, TNF-α, and MCP-1), post-challenge. Enhanced T-cell epitope recruitment post-challenge was seen in the groups that received Advax™ adjuvant. Immunisation with GNP-LLO91-99 plus Advax™ adjuvant provided equally robust Listeria protection as the best DC vaccine strategy but without the complexity and cost, making this a highly promising strategy for development of a prophylactic vaccine against listeriosis. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 650 | 4 | |a Bacterial Toxins - immunology | |
| 650 | 4 | |a Bacterial Vaccines - immunology | |
| 650 | 4 | |a Heat-Shock Proteins - immunology | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Cytokines - immunology | |
| 650 | 4 | |a Inulin - immunology | |
| 650 | 4 | |a Spleen - cytology | |
| 650 | 4 | |a Cytokines - metabolism | |
| 650 | 4 | |a Listeriosis - prevention & control | |
| 650 | 4 | |a T-Lymphocytes - immunology | |
| 650 | 4 | |a CD4-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Hemolysin Proteins - immunology | |
| 650 | 4 | |a Dendritic Cells - immunology | |
| 650 | 4 | |a Epitopes, T-Lymphocyte - immunology | |
| 650 | 4 | |a Listeria monocytogenes - immunology | |
| 650 | 4 | |a Ctsd | |
| 650 | 4 | |a Apoptosis | |
| 650 | 4 | |a colony forming units | |
| 650 | 4 | |a gold glyconanoparticles | |
| 650 | 4 | |a Glucose | |
| 650 | 4 | |a i.v | |
| 650 | 4 | |a Lymphocytes | |
| 650 | 4 | |a intra-peritoneal | |
| 650 | 4 | |a CFU | |
| 650 | 4 | |a Listeria | |
| 650 | 4 | |a i.p | |
| 650 | 4 | |a intra-venous | |
| 650 | 4 | |a cathepsin-D | |
| 650 | 4 | |a MIIC | |
| 650 | 4 | |a MHC-class II antigen loading compartments | |
| 650 | 4 | |a macrophages | |
| 650 | 4 | |a APC | |
| 650 | 4 | |a major histocompatibility complex | |
| 650 | 4 | |a antigen-presenting cells | |
| 650 | 4 | |a Listeria monocytogenes | |
| 650 | 4 | |a Biocompatibility | |
| 650 | 4 | |a Abbreviations | |
| 650 | 4 | |a dendritic cells | |
| 650 | 4 | |a MHC | |
| 650 | 4 | |a Hepatitis | |
| 650 | 4 | |a GNP | |
| 650 | 4 | |a Peptides | |
| 650 | 4 | |a Vaccines | |
| 650 | 4 | |a LLO | |
| 650 | 4 | |a T cell receptors | |
| 650 | 4 | |a Ligands | |
| 650 | 4 | |a West Nile virus | |
| 650 | 4 | |a listeriolysin O | |
| 700 | 1 | |a Marradi, Marco |4 oth | |
| 700 | 1 | |a Calderon-Gonzalez, Ricardo |4 oth | |
| 700 | 1 | |a Frande-Cabanes, Elisabet |4 oth | |
| 700 | 1 | |a Penadés, Soledad |4 oth | |
| 700 | 1 | |a Petrovsky, Nikolai |4 oth | |
| 700 | 1 | |a Alvarez-Dominguez, Carmen |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Vaccine |d Amsterdam : Elsevier, 1983 |g 33(2015), 12, Seite 1465-1473 |w (DE-627)130402427 |w (DE-600)605674-X |w (DE-576)015904938 |x 0264-410X |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2015 |g number:12 |g pages:1465-1473 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.vaccine.2015.01.062 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25659269 |
| 856 | 4 | 2 | |u http://search.proquest.com/docview/1664536864 |
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