Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant
Riemerella anatipestifer, a Gram-negative bacillus, causes septicemia that can result in high mortality for ducklings. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant outer membrane protein A (rOmpA) and plasmid constructs containing CpG oligodeoxynucleotides (ODN). Results showed that CpG ODN enhanced both humoral and cell-mediated immunity elicited by rOmpA as early as two weeks after primary immunization. When compared to ducks immunized with rOmpA, ducks immunized with rOmpA+CpG ODN showed higher levels (p<0.05) of antibody titer, T cell proliferation, and percentages of CD4(+) and CD8(+) T cell in peripheral blood mononuclear cells (PBMCs). The relative fold inductions of mRNA expression of Th1-type (IFN-γ and IL-12), and Th2-type (IL-6) cytokines in PBMCs isolated from ducks immunized with rOmpA+CpG ODN were significantly higher than those of the rOmpA group. Homologous challenge result showed that the rOmpA+CpG ODN vaccine reduced the pathological score by 90% in comparison with the saline control. In conclusion, our study found that CpG ODN can enhance both humoral and cellular immunity elicited by a rOmpA vaccine. The rOmpA+CpG ODN vaccine can be further developed as a subunit vaccine against R. anatipestifer..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Vaccine - 33(2015), 1, Seite 92-99 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chu, Chun-Yen [VerfasserIn] |
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| Links: |
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| doi: |
10.1016/j.vaccine.2014.11.010 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC1956703179 |
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| 100 | 1 | |a Chu, Chun-Yen |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant |
| 264 | 1 | |c 2015 | |
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| 520 | |a Riemerella anatipestifer, a Gram-negative bacillus, causes septicemia that can result in high mortality for ducklings. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant outer membrane protein A (rOmpA) and plasmid constructs containing CpG oligodeoxynucleotides (ODN). Results showed that CpG ODN enhanced both humoral and cell-mediated immunity elicited by rOmpA as early as two weeks after primary immunization. When compared to ducks immunized with rOmpA, ducks immunized with rOmpA+CpG ODN showed higher levels (p<0.05) of antibody titer, T cell proliferation, and percentages of CD4(+) and CD8(+) T cell in peripheral blood mononuclear cells (PBMCs). The relative fold inductions of mRNA expression of Th1-type (IFN-γ and IL-12), and Th2-type (IL-6) cytokines in PBMCs isolated from ducks immunized with rOmpA+CpG ODN were significantly higher than those of the rOmpA group. Homologous challenge result showed that the rOmpA+CpG ODN vaccine reduced the pathological score by 90% in comparison with the saline control. In conclusion, our study found that CpG ODN can enhance both humoral and cellular immunity elicited by a rOmpA vaccine. The rOmpA+CpG ODN vaccine can be further developed as a subunit vaccine against R. anatipestifer. | ||
| 540 | |a Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. | ||
| 650 | 4 | |a Recombinant Proteins - genetics | |
| 650 | 4 | |a Bacterial Vaccines - immunology | |
| 650 | 4 | |a Flavobacteriaceae Infections - veterinary | |
| 650 | 4 | |a Riemerella - immunology | |
| 650 | 4 | |a Flavobacteriaceae Infections - pathology | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Adjuvants, Immunologic - administration & dosage | |
| 650 | 4 | |a Bacterial Vaccines - genetics | |
| 650 | 4 | |a Bacterial Vaccines - administration & dosage | |
| 650 | 4 | |a Vaccines, Subunit - administration & dosage | |
| 650 | 4 | |a Bacterial Outer Membrane Proteins - immunology | |
| 650 | 4 | |a Oligodeoxyribonucleotides - administration & dosage | |
| 650 | 4 | |a Vaccines, Subunit - immunology | |
| 650 | 4 | |a Riemerella - genetics | |
| 650 | 4 | |a Poultry Diseases - prevention & control | |
| 650 | 4 | |a Vaccines, Subunit - genetics | |
| 650 | 4 | |a Bacterial Outer Membrane Proteins - genetics | |
| 650 | 4 | |a Antibodies, Bacterial - blood | |
| 650 | 4 | |a CD4-Positive T-Lymphocytes - immunology | |
| 650 | 4 | |a Flavobacteriaceae Infections - prevention & control | |
| 650 | 4 | |a Recombinant Proteins - immunology | |
| 650 | 4 | |a Cytokines - biosynthesis | |
| 650 | 4 | |a Poultry Diseases - pathology | |
| 700 | 1 | |a Liu, Chia-Hui |4 oth | |
| 700 | 1 | |a Liou, Jhong-Jie |4 oth | |
| 700 | 1 | |a Lee, Jai-Wei |4 oth | |
| 700 | 1 | |a Cheng, Li-Ting |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Vaccine |d Amsterdam : Elsevier, 1983 |g 33(2015), 1, Seite 92-99 |w (DE-627)130402427 |w (DE-600)605674-X |w (DE-576)015904938 |x 0264-410X |7 nnns |
| 773 | 1 | 8 | |g volume:33 |g year:2015 |g number:1 |g pages:92-99 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1016/j.vaccine.2014.11.010 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25448104 |
| 912 | |a GBV_USEFLAG_A | ||
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| 912 | |a GBV_ILN_73 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4219 | ||
| 951 | |a AR | ||
| 952 | |d 33 |j 2015 |e 1 |h 92-99 | ||