Characterizing fitness and immune escape of SARS-CoV-2 EG.5 sublineage using elderly serum and nasal organoid
© 2024 The Author(s)..
SARS-CoV-2 Omicron variant has evolved into sublineages. Here, we compared the neutralization susceptibility and viral fitness of EG.5.1 and XBB.1.9.1. Serum neutralization antibody titer against EG.5.1 was 1.71-fold lower than that for XBB.1.9.1. However, there was no significant difference in virus replication between EG.5.1 and XBB.1.9.1 in human nasal organoids and TMPRSS2/ACE2 over-expressing A549 cells. No significant difference was observed in competitive fitness and cytokine/chemokine response between EG.5.1 and XBB.1.9.1. Both EG.5.1 and XBB.1.9.1 replicated more robustly in the nasal organoid from a younger adult than that from an older adult. Our findings suggest that enhanced immune escape contributes to the dominance of EG.5.1 over earlier sublineages. The combination of population serum susceptibility testing and viral fitness evaluation with nasal organoids may hold promise in risk assessment of upcoming variants. Utilization of serum specimens and nasal organoid derived from older adults provides a targeted risk assessment for this vulnerable population.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
iScience - 27(2024), 5 vom: 17. Apr., Seite 109706 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Xiaojuan [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Revised 26.04.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.isci.2024.109706 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM371492785 |
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| 520 | |a SARS-CoV-2 Omicron variant has evolved into sublineages. Here, we compared the neutralization susceptibility and viral fitness of EG.5.1 and XBB.1.9.1. Serum neutralization antibody titer against EG.5.1 was 1.71-fold lower than that for XBB.1.9.1. However, there was no significant difference in virus replication between EG.5.1 and XBB.1.9.1 in human nasal organoids and TMPRSS2/ACE2 over-expressing A549 cells. No significant difference was observed in competitive fitness and cytokine/chemokine response between EG.5.1 and XBB.1.9.1. Both EG.5.1 and XBB.1.9.1 replicated more robustly in the nasal organoid from a younger adult than that from an older adult. Our findings suggest that enhanced immune escape contributes to the dominance of EG.5.1 over earlier sublineages. The combination of population serum susceptibility testing and viral fitness evaluation with nasal organoids may hold promise in risk assessment of upcoming variants. Utilization of serum specimens and nasal organoid derived from older adults provides a targeted risk assessment for this vulnerable population | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Immune response | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Virology | |
| 700 | 1 | |a Lam, Stephanie Joy-Ann |e verfasserin |4 aut | |
| 700 | 1 | |a Ip, Jonathan Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Fong, Carol Ho-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Allen Wing-Ho |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Wan-Mui |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Yoyo Suet-Yiu |e verfasserin |4 aut | |
| 700 | 1 | |a Tsoi, Hoi-Wah |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Brian Pui-Chun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Lin-Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Meng, Xinjie |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Shuofeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Hanjun |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Vincent Chi-Chung |e verfasserin |4 aut | |
| 700 | 1 | |a Yuen, Jacqueline Kwan Yuk |e verfasserin |4 aut | |
| 700 | 1 | |a Yuen, Kwok-Yung |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a To, Kelvin Kai-Wang |e verfasserin |4 aut | |
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