Identification of marker genes associated with N6-methyladenosine and autophagy in ulcerative colitis
©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved..
BACKGROUND: Both N6-methyladenosine (m6A) methylation and autophagy are considered relevant to the pathogenesis of ulcerative colitis (UC). However, a systematic exploration of the role of the com-bination of m6A methylation and autophagy in UC remains to be performed.
AIM: To elucidate the autophagy-related genes of m6A with a diagnostic value for UC.
METHODS: The correlation between m6A-related genes and autophagy-related genes (ARGs) was analyzed. Finally, gene set enrichment analysis (GSEA) was performed on the characteristic genes. Additionally, the expression levels of four characteristic genes were verified in dextran sulfate sodium (DSS)-induced colitis in mice.
RESULTS: GSEA indicated that BAG3, P4HB and TP53INP2 were involved in the inflammatory response and TNF-α signalling via nuclear factor kappa-B. Furthermore, polymerase chain reaction results showed significantly higher mRNA levels of BAG3 and P4HB and lower mRNA levels of FMR1 and TP53INP2 in the DSS group compared to the control group.
CONCLUSION: This study identified four m6A-ARGs that predict the occurrence of UC, thus providing a scientific reference for further studies on the pathogenesis of UC.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
|---|---|
| Enthalten in: |
World journal of clinical cases - 12(2024), 10 vom: 06. Apr., Seite 1750-1765 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Liu, Xiao-Yan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Autophagy |
|---|
| Anmerkungen: |
Date Revised 26.04.2024 published: Print Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.12998/wjcc.v12.i10.1750 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM371489571 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM371489571 | ||
| 003 | DE-627 | ||
| 005 | 20240426235211.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240426s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.12998/wjcc.v12.i10.1750 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1388.xml |
| 035 | |a (DE-627)NLM371489571 | ||
| 035 | |a (NLM)38660076 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Liu, Xiao-Yan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of marker genes associated with N6-methyladenosine and autophagy in ulcerative colitis |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 26.04.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Both N6-methyladenosine (m6A) methylation and autophagy are considered relevant to the pathogenesis of ulcerative colitis (UC). However, a systematic exploration of the role of the com-bination of m6A methylation and autophagy in UC remains to be performed | ||
| 520 | |a AIM: To elucidate the autophagy-related genes of m6A with a diagnostic value for UC | ||
| 520 | |a METHODS: The correlation between m6A-related genes and autophagy-related genes (ARGs) was analyzed. Finally, gene set enrichment analysis (GSEA) was performed on the characteristic genes. Additionally, the expression levels of four characteristic genes were verified in dextran sulfate sodium (DSS)-induced colitis in mice | ||
| 520 | |a RESULTS: GSEA indicated that BAG3, P4HB and TP53INP2 were involved in the inflammatory response and TNF-α signalling via nuclear factor kappa-B. Furthermore, polymerase chain reaction results showed significantly higher mRNA levels of BAG3 and P4HB and lower mRNA levels of FMR1 and TP53INP2 in the DSS group compared to the control group | ||
| 520 | |a CONCLUSION: This study identified four m6A-ARGs that predict the occurrence of UC, thus providing a scientific reference for further studies on the pathogenesis of UC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Autophagy | |
| 650 | 4 | |a Characteristic genes | |
| 650 | 4 | |a Immune infiltration | |
| 650 | 4 | |a Ulcerative colitis | |
| 650 | 4 | |a m6A | |
| 700 | 1 | |a Qiao, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ya-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zi-Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, You-Lan |e verfasserin |4 aut | |
| 700 | 1 | |a Que, Ren-Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Hong-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Yan-Cheng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t World journal of clinical cases |d 2013 |g 12(2024), 10 vom: 06. Apr., Seite 1750-1765 |w (DE-627)NLM233331727 |x 2307-8960 |7 nnns |
| 773 | 1 | 8 | |g volume:12 |g year:2024 |g number:10 |g day:06 |g month:04 |g pages:1750-1765 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.12998/wjcc.v12.i10.1750 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 12 |j 2024 |e 10 |b 06 |c 04 |h 1750-1765 | ||