Optogenetically engineered calcium oscillations promote autophagy-mediated cell death via AMPK activation
Autophagy is a double-edged sword for cells; it can lead to both cell survival and death. Calcium (Ca2+) signalling plays a crucial role in regulating various cellular behaviours, including cell migration, proliferation and death. In this study, we investigated the effects of modulating cytosolic Ca2+ levels on autophagy using chemical and optogenetic methods. Our findings revealed that ionomycin and thapsigargin induce Ca2+ influx to promote autophagy, whereas the Ca2+ chelator BAPTA-AM induces Ca2+ depletion and inhibits autophagy. Furthermore, the optogenetic platform allows the manipulation of illumination parameters, including density, frequency, duty cycle and duration, to create different patterns of Ca2+ oscillations. We used the optogenetic tool Ca2+-translocating channelrhodopsin, which is activated and opened by 470 nm blue light to induce Ca2+ influx. These results demonstrated that high-frequency Ca2+ oscillations induce autophagy. In addition, autophagy induction may involve Ca2+-activated adenosine monophosphate (AMP)-activated protein kinases. In conclusion, high-frequency optogenetic Ca2+ oscillations led to cell death mediated by AMP-activated protein kinase-induced autophagy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Open biology - 14(2024), 4 vom: 23. Apr., Seite 240001 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lai, Yi-Shyun [VerfasserIn] |
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| Links: |
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| Themen: |
56092-81-0 |
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| Anmerkungen: |
Date Completed 24.04.2024 Date Revised 01.05.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1098/rsob.240001 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM37142237X |
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| 245 | 1 | 0 | |a Optogenetically engineered calcium oscillations promote autophagy-mediated cell death via AMPK activation |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Autophagy is a double-edged sword for cells; it can lead to both cell survival and death. Calcium (Ca2+) signalling plays a crucial role in regulating various cellular behaviours, including cell migration, proliferation and death. In this study, we investigated the effects of modulating cytosolic Ca2+ levels on autophagy using chemical and optogenetic methods. Our findings revealed that ionomycin and thapsigargin induce Ca2+ influx to promote autophagy, whereas the Ca2+ chelator BAPTA-AM induces Ca2+ depletion and inhibits autophagy. Furthermore, the optogenetic platform allows the manipulation of illumination parameters, including density, frequency, duty cycle and duration, to create different patterns of Ca2+ oscillations. We used the optogenetic tool Ca2+-translocating channelrhodopsin, which is activated and opened by 470 nm blue light to induce Ca2+ influx. These results demonstrated that high-frequency Ca2+ oscillations induce autophagy. In addition, autophagy induction may involve Ca2+-activated adenosine monophosphate (AMP)-activated protein kinases. In conclusion, high-frequency optogenetic Ca2+ oscillations led to cell death mediated by AMP-activated protein kinase-induced autophagy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a AMPK | |
| 650 | 4 | |a autophagy | |
| 650 | 4 | |a calcium | |
| 650 | 4 | |a cell death | |
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| 700 | 1 | |a Nguyen, Thi My Hang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Ying-Chi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hsueh-Chun |e verfasserin |4 aut | |
| 700 | 1 | |a Chiu, Wen-Tai |e verfasserin |4 aut | |
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