Pan-microscopic examination of monkeypox virus in trophoblasts cells reveals new insights into virions release through filopodia-like projections
© 2024 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC..
Vertical transmission has been described following monkeypox virus (MPXV) infection in pregnant women. The presence of MPXV has been reported in the placenta from infected women, but whether pathogens colonize placenta remains unexplored. We identify trophoblasts as a target cell for MPXV replication. In a pan-microscopy approach, we decipher the specific infectious cycle of MPXV and inner cellular structures in trophoblasts. We identified the formation of a specialized region for viral morphogenesis and replication in placental cells. We also reported infection-induced cellular remodeling. We found that MPXV stimulates cytoskeleton reorganization with intercellular extensions for MPXV cell spreading specifically to trophoblastic cells. Altogether, the specific infectious cycle of MPXV in trophoblast cells and these protrusions that were structurally and morphologically similar to filopodia reveal new insights into the infection of MPXV.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:96 |
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| Enthalten in: |
Journal of medical virology - 96(2024), 4 vom: 30. Apr., Seite e29620 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Andrieu, Jonatane [VerfasserIn] |
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| Links: |
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| Themen: |
Infectious diseases |
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| Anmerkungen: |
Date Completed 29.04.2024 Date Revised 29.04.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/jmv.29620 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM371359996 |
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| 520 | |a © 2024 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC. | ||
| 520 | |a Vertical transmission has been described following monkeypox virus (MPXV) infection in pregnant women. The presence of MPXV has been reported in the placenta from infected women, but whether pathogens colonize placenta remains unexplored. We identify trophoblasts as a target cell for MPXV replication. In a pan-microscopy approach, we decipher the specific infectious cycle of MPXV and inner cellular structures in trophoblasts. We identified the formation of a specialized region for viral morphogenesis and replication in placental cells. We also reported infection-induced cellular remodeling. We found that MPXV stimulates cytoskeleton reorganization with intercellular extensions for MPXV cell spreading specifically to trophoblastic cells. Altogether, the specific infectious cycle of MPXV in trophoblast cells and these protrusions that were structurally and morphologically similar to filopodia reveal new insights into the infection of MPXV | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 650 | 4 | |a monkeypox virus | |
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| 650 | 4 | |a poxvirus | |
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| 650 | 4 | |a trophoblast | |
| 700 | 1 | |a Valade, Margaux |e verfasserin |4 aut | |
| 700 | 1 | |a Lebideau, Marion |e verfasserin |4 aut | |
| 700 | 1 | |a Bretelle, Florence |e verfasserin |4 aut | |
| 700 | 1 | |a Mège, Jean-Louis |e verfasserin |4 aut | |
| 700 | 1 | |a Wurtz, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Mezouar, Soraya |e verfasserin |4 aut | |
| 700 | 1 | |a La Scola, Bernard |e verfasserin |4 aut | |
| 700 | 1 | |a Baudoin, Jean-Pierre |e verfasserin |4 aut | |
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