Designing a size exclusion-based hapten and the development of a quantitative and visual time-resolved fluorescence immunochromatography assay strip for detecting dimethomorph and flumorph in a group-specific manner
Copyright © 2023. Published by Elsevier Ltd..
Based on the size and surface properties of dimethomorph and flumorph, we used a computer simulation-assisted size exclusion hapten design strategy to develop group-specific monoclonal antibodies that can simultaneously recognize dimethomorph and flumorph. For this, we performed quantitative and visual semi-quantitative time-resolved fluorescence immunochromatography (TRFICA) to simultaneously detect dimethomorph and flumorph in potatoes and apples. In potato samples, the visual limit of detection (vLOD) for dimethomorph and flumorph was 4 ng/mL and 8 ng/mL, respectively, whereas the quantitative limit of detection (qLOD) for dimethomorph and flumorph was 0.26 and 0.33 ng/mL, respectively. The vLOD of dimethomorph and flumorph in apple samples was 8 ng/mL, whereas the qLOD of dimethomorph and flumorph was 0.17 and 0.38 ng/mL, respectively. The average recovery of potato and apple samples ranged from 77.5% to 121.7%, which indicated that the method can be used to rapidly detect dimethomorph and flumorph in food samples.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:450 |
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| Enthalten in: |
Food chemistry - 450(2024) vom: 15. Apr., Seite 139372 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhu, Yuxian [VerfasserIn] |
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| Links: |
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| Themen: |
Apple |
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| Anmerkungen: |
Date Revised 19.04.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.foodchem.2024.139372 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2023. Published by Elsevier Ltd. | ||
| 520 | |a Based on the size and surface properties of dimethomorph and flumorph, we used a computer simulation-assisted size exclusion hapten design strategy to develop group-specific monoclonal antibodies that can simultaneously recognize dimethomorph and flumorph. For this, we performed quantitative and visual semi-quantitative time-resolved fluorescence immunochromatography (TRFICA) to simultaneously detect dimethomorph and flumorph in potatoes and apples. In potato samples, the visual limit of detection (vLOD) for dimethomorph and flumorph was 4 ng/mL and 8 ng/mL, respectively, whereas the quantitative limit of detection (qLOD) for dimethomorph and flumorph was 0.26 and 0.33 ng/mL, respectively. The vLOD of dimethomorph and flumorph in apple samples was 8 ng/mL, whereas the qLOD of dimethomorph and flumorph was 0.17 and 0.38 ng/mL, respectively. The average recovery of potato and apple samples ranged from 77.5% to 121.7%, which indicated that the method can be used to rapidly detect dimethomorph and flumorph in food samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Apple | |
| 650 | 4 | |a Dimethomorph | |
| 650 | 4 | |a Flumorph | |
| 650 | 4 | |a Potato | |
| 650 | 4 | |a Time-resolved fluorescence immunochromatographic assay (TRFICA) | |
| 700 | 1 | |a Zhang, Yongyi |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Daoping |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Haoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jinyi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Zhenlin |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yuanming |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Yuanxin |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Yudong |e verfasserin |4 aut | |
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