A fine-scale Arabidopsis chromatin landscape reveals chromatin conformation-associated transcriptional dynamics
© 2024. The Author(s)..
Plants, as sessile organisms, deploy transcriptional dynamics for adapting to extreme growth conditions such as cold stress. Emerging evidence suggests that chromatin architecture contributes to transcriptional regulation. However, the relationship between chromatin architectural dynamics and transcriptional reprogramming in response to cold stress remains unclear. Here, we apply a chemical-crosslinking assisted proximity capture (CAP-C) method to elucidate the fine-scale chromatin landscape, revealing chromatin interactions within gene bodies closely associated with RNA polymerase II (Pol II) densities across initiation, pausing, and termination sites. We observe dynamic changes in chromatin interactions alongside Pol II activity alterations during cold stress, suggesting local chromatin dynamics may regulate Pol II activity. Notably, cold stress does not affect large-scale chromatin conformations. We further identify a comprehensive promoter-promoter interaction (PPI) network across the genome, potentially facilitating co-regulation of gene expression in response to cold stress. Our study deepens the understanding of chromatin conformation-associated gene regulation in plant response to cold.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Nature communications - 15(2024), 1 vom: 16. Apr., Seite 3253 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Yueying [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.04.2024 Date Revised 25.04.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-024-47678-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM371165032 |
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| 245 | 1 | 2 | |a A fine-scale Arabidopsis chromatin landscape reveals chromatin conformation-associated transcriptional dynamics |
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| 520 | |a © 2024. The Author(s). | ||
| 520 | |a Plants, as sessile organisms, deploy transcriptional dynamics for adapting to extreme growth conditions such as cold stress. Emerging evidence suggests that chromatin architecture contributes to transcriptional regulation. However, the relationship between chromatin architectural dynamics and transcriptional reprogramming in response to cold stress remains unclear. Here, we apply a chemical-crosslinking assisted proximity capture (CAP-C) method to elucidate the fine-scale chromatin landscape, revealing chromatin interactions within gene bodies closely associated with RNA polymerase II (Pol II) densities across initiation, pausing, and termination sites. We observe dynamic changes in chromatin interactions alongside Pol II activity alterations during cold stress, suggesting local chromatin dynamics may regulate Pol II activity. Notably, cold stress does not affect large-scale chromatin conformations. We further identify a comprehensive promoter-promoter interaction (PPI) network across the genome, potentially facilitating co-regulation of gene expression in response to cold stress. Our study deepens the understanding of chromatin conformation-associated gene regulation in plant response to cold | ||
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| 700 | 1 | |a Dong, Qianli |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qinzhe |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Haopeng |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Wenqing |e verfasserin |4 aut | |
| 700 | 1 | |a Cheema, Jitender |e verfasserin |4 aut | |
| 700 | 1 | |a You, Qiancheng |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Xiaofeng |e verfasserin |4 aut | |
| 700 | 1 | |a He, Chuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Yiliang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Huakun |e verfasserin |4 aut | |
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