Multiwalled Carbon Nanotubes-Reprogrammed Macrophages Facilitate Breast Cancer Metastasis via NBR2/TBX1 Axis
In recent years, carbon nanotubes have emerged as a widely used nanomaterial, but their human exposure has become a significant concern. In our former study, we reported that pulmonary exposure of multiwalled carbon nanotubes (MWCNTs) promoted tumor metastasis of breast cancer; macrophages were key effectors of MWCNTs and contributed to the metastasis-promoting procedure in breast cancer, but the underlying molecular mechanisms remain to be explored. As a follow-up study, we herein demonstrated that MWCNT exposure in breast cancer cells and macrophage coculture systems promoted metastasis of breast cancer cells both in vitro and in vivo; macrophages were skewed into M2 polarization by MWCNT exposure. LncRNA NBR2 was screened out to be significantly decreased in MWCNTs-stimulated macrophages through RNA-seq; depletion of NBR2 led to the acquisition of M2 phenotypes in macrophages by activating multiple M2-related pathways. Specifically, NBR2 was found to positively regulate the downstream gene TBX1 through H3k27ac activation. TBX1 silence rescued NBR2-induced impairment of M2 polarization in IL-4 & IL-13-stimulated macrophages. Moreover, NBR2 overexpression mitigated the enhancing effects of MWCNT-exposed macrophages on breast cancer metastasis. This study uncovered the molecular mechanisms underlying breast cancer metastasis induced by MWCNT exposure.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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Enthalten in: |
ACS nano - (2024) vom: 16. Apr. |
Sprache: |
Englisch |
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Beteiligte Personen: |
Ding, Keshuo [VerfasserIn] |
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H3k27ac |
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Date Revised 16.04.2024 published: Print-Electronic Citation Status Publisher |
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doi: |
10.1021/acsnano.3c11651 |
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PPN (Katalog-ID): |
NLM371129273 |
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520 | |a In recent years, carbon nanotubes have emerged as a widely used nanomaterial, but their human exposure has become a significant concern. In our former study, we reported that pulmonary exposure of multiwalled carbon nanotubes (MWCNTs) promoted tumor metastasis of breast cancer; macrophages were key effectors of MWCNTs and contributed to the metastasis-promoting procedure in breast cancer, but the underlying molecular mechanisms remain to be explored. As a follow-up study, we herein demonstrated that MWCNT exposure in breast cancer cells and macrophage coculture systems promoted metastasis of breast cancer cells both in vitro and in vivo; macrophages were skewed into M2 polarization by MWCNT exposure. LncRNA NBR2 was screened out to be significantly decreased in MWCNTs-stimulated macrophages through RNA-seq; depletion of NBR2 led to the acquisition of M2 phenotypes in macrophages by activating multiple M2-related pathways. Specifically, NBR2 was found to positively regulate the downstream gene TBX1 through H3k27ac activation. TBX1 silence rescued NBR2-induced impairment of M2 polarization in IL-4 & IL-13-stimulated macrophages. Moreover, NBR2 overexpression mitigated the enhancing effects of MWCNT-exposed macrophages on breast cancer metastasis. This study uncovered the molecular mechanisms underlying breast cancer metastasis induced by MWCNT exposure | ||
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700 | 1 | |a Zhu, Linyan |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Tian-Tian |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Rumeng |e verfasserin |4 aut | |
700 | 1 | |a Li, Qiushuang |e verfasserin |4 aut | |
700 | 1 | |a Xie, Bin |e verfasserin |4 aut | |
700 | 1 | |a Ding, Lin |e verfasserin |4 aut | |
700 | 1 | |a Shang, Limeng |e verfasserin |4 aut | |
700 | 1 | |a Wang, Yi |e verfasserin |4 aut | |
700 | 1 | |a Xu, Panpan |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Tao |e verfasserin |4 aut | |
700 | 1 | |a Chen, Chunying |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Yong |e verfasserin |4 aut | |
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