Syringic Acid Attenuates the IL-1β-Induced Akt Pathway in Chondrocyte ATDC5 Cells
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net..
BACKGROUND: Osteoarthritis (OA) is a chronic progressive joint ailment that is largely predominant worldwide. However, it typically gets worse over time, occurs more frequently, and becomes more crippling.
OBJECTIVES: Syringic acid (SA) is a well-known phenolic compound reported to suppress inflammation, cell proliferation, and apoptosis of various cancer cells. Since the role of SA in OA remains unknown, there is a need to hypothesize the anti-inflammatory activities of SA on IL- 1β-induced ATDC5 chondrocyte‑like cells and to elucidate its protective action against OA.
METHODS: The cytotoxicity, inflammatory mediators, mRNA expression of MMPs, ADAMTS, COX-2, and Akt/NF-κB protein expression of SA activity on ATDC5 cells were examined through CCK-8 assay, ELISA, RT-qPCR, and western blot. It was found that SA (10, 20, and 30 μM) did not show any inhibitory effects on the viability of the ATDC5 cells in a concentrationdependent manner.
RESULTS: SA markedly reduced the inflammatory mediators, cytokines, PGE2, MMPs, COX-2, and ADAMTS in a concentration-dependent manner. Likewise, SA expressively attenuated IL- 1β-stimulated Akt phosphorylation and NF-κB activation as well as IL-1β- induced ATDC5 chondrocytes.
CONCLUSION: This study revealed that SA is a novel candidate applicable for the treatment of OA.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Combinatorial chemistry & high throughput screening - (2024) vom: 08. Apr. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
He, Xiao-Feng [VerfasserIn] |
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| Links: |
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| Themen: |
ATDC5 cells |
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| Anmerkungen: |
Date Revised 15.04.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.2174/0113862073286384240227053954 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM371060885 |
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| 245 | 1 | 0 | |a Syringic Acid Attenuates the IL-1β-Induced Akt Pathway in Chondrocyte ATDC5 Cells |
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| 500 | |a Date Revised 15.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net. | ||
| 520 | |a BACKGROUND: Osteoarthritis (OA) is a chronic progressive joint ailment that is largely predominant worldwide. However, it typically gets worse over time, occurs more frequently, and becomes more crippling | ||
| 520 | |a OBJECTIVES: Syringic acid (SA) is a well-known phenolic compound reported to suppress inflammation, cell proliferation, and apoptosis of various cancer cells. Since the role of SA in OA remains unknown, there is a need to hypothesize the anti-inflammatory activities of SA on IL- 1β-induced ATDC5 chondrocyte‑like cells and to elucidate its protective action against OA | ||
| 520 | |a METHODS: The cytotoxicity, inflammatory mediators, mRNA expression of MMPs, ADAMTS, COX-2, and Akt/NF-κB protein expression of SA activity on ATDC5 cells were examined through CCK-8 assay, ELISA, RT-qPCR, and western blot. It was found that SA (10, 20, and 30 μM) did not show any inhibitory effects on the viability of the ATDC5 cells in a concentrationdependent manner | ||
| 520 | |a RESULTS: SA markedly reduced the inflammatory mediators, cytokines, PGE2, MMPs, COX-2, and ADAMTS in a concentration-dependent manner. Likewise, SA expressively attenuated IL- 1β-stimulated Akt phosphorylation and NF-κB activation as well as IL-1β- induced ATDC5 chondrocytes | ||
| 520 | |a CONCLUSION: This study revealed that SA is a novel candidate applicable for the treatment of OA | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ATDC5 cells | |
| 650 | 4 | |a Akt/NF-κB. | |
| 650 | 4 | |a MMPs | |
| 650 | 4 | |a Syringic acid | |
| 650 | 4 | |a osteoarthritis | |
| 700 | 1 | |a Xiong, Zhi-Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Qing-Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Lei, Yi-Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Vijayalakshmi, Annamali |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Gang |e verfasserin |4 aut | |
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