Metabolomic changes following GenX and PFBS exposure in developing zebrafish
Copyright © 2024 Elsevier B.V. All rights reserved..
Short chain per- and polyfluoroalkyl substances (PFAS), including hexafluoropropylene oxide dimer acid (GenX) and perfluorobutane sulfonate (PFBS), are replacement chemicals for environmentally persistent, long-chain PFAS. Although GenX and PFBS have been detected in surface and ground water worldwide, few studies provide information on the metabolic alterations or risks associated with their exposures. In this study, larval zebrafish were used to investigate the toxicity of early-life exposure to GenX or PFBS. Zebrafish were chronically exposed from 4 h post-fertilization (hpf) to 6 days post-fertilization (dpf) to 150 µM GenX or 95.0 µM PFBS. Ultra-high-performance liquid chromatography paired with high-resolution mass spectrometry was used to quantify uptake of GenX and PFBS into zebrafish larvae and perform targeted and untargeted metabolomics. Our results indicate that PFBS was 20.4 % more readily absorbed into the zebrafish larvae compared to GenX. Additionally, PFBS exposure significantly altered 13 targeted metabolites and 21 metabolic pathways, while GenX exposure significantly altered 1 targeted metabolite and 17 metabolic pathways. Exposure to GenX, and to an even greater extent PFBS, resulted in a number of altered metabolic pathways in the amino acid metabolism, with other significant alterations in the carbohydrate, lipid, cofactors and vitamins, nucleotide, and xenobiotics metabolisms. Our results indicate that GenX and PFBS impact the zebrafish metabolome, with implications of global metabolic dysregulation, particularly in metabolic pathways relating to growth and development.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:271 |
|---|---|
| Enthalten in: |
Aquatic toxicology (Amsterdam, Netherlands) - 271(2024) vom: 03. Apr., Seite 106908 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dunn, Fiona [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Body burden |
|---|
| Anmerkungen: |
Date Revised 12.04.2024 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1016/j.aquatox.2024.106908 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370978803 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370978803 | ||
| 003 | DE-627 | ||
| 005 | 20240413233035.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240413s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.aquatox.2024.106908 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1374.xml |
| 035 | |a (DE-627)NLM370978803 | ||
| 035 | |a (NLM)38608566 | ||
| 035 | |a (PII)S0166-445X(24)00078-X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Dunn, Fiona |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Metabolomic changes following GenX and PFBS exposure in developing zebrafish |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 12.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a Short chain per- and polyfluoroalkyl substances (PFAS), including hexafluoropropylene oxide dimer acid (GenX) and perfluorobutane sulfonate (PFBS), are replacement chemicals for environmentally persistent, long-chain PFAS. Although GenX and PFBS have been detected in surface and ground water worldwide, few studies provide information on the metabolic alterations or risks associated with their exposures. In this study, larval zebrafish were used to investigate the toxicity of early-life exposure to GenX or PFBS. Zebrafish were chronically exposed from 4 h post-fertilization (hpf) to 6 days post-fertilization (dpf) to 150 µM GenX or 95.0 µM PFBS. Ultra-high-performance liquid chromatography paired with high-resolution mass spectrometry was used to quantify uptake of GenX and PFBS into zebrafish larvae and perform targeted and untargeted metabolomics. Our results indicate that PFBS was 20.4 % more readily absorbed into the zebrafish larvae compared to GenX. Additionally, PFBS exposure significantly altered 13 targeted metabolites and 21 metabolic pathways, while GenX exposure significantly altered 1 targeted metabolite and 17 metabolic pathways. Exposure to GenX, and to an even greater extent PFBS, resulted in a number of altered metabolic pathways in the amino acid metabolism, with other significant alterations in the carbohydrate, lipid, cofactors and vitamins, nucleotide, and xenobiotics metabolisms. Our results indicate that GenX and PFBS impact the zebrafish metabolome, with implications of global metabolic dysregulation, particularly in metabolic pathways relating to growth and development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Body burden | |
| 650 | 4 | |a Embryonic development | |
| 650 | 4 | |a GenX | |
| 650 | 4 | |a Metabolomics | |
| 650 | 4 | |a PFBS | |
| 650 | 4 | |a Zebrafish | |
| 700 | 1 | |a Paquette, Shannon E |e verfasserin |4 aut | |
| 700 | 1 | |a Pennell, Kurt D |e verfasserin |4 aut | |
| 700 | 1 | |a Plavicki, Jessica S |e verfasserin |4 aut | |
| 700 | 1 | |a Manz, Katherine E |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Aquatic toxicology (Amsterdam, Netherlands) |d 1993 |g 271(2024) vom: 03. Apr., Seite 106908 |w (DE-627)NLM08474832X |x 1879-1514 |7 nnns |
| 773 | 1 | 8 | |g volume:271 |g year:2024 |g day:03 |g month:04 |g pages:106908 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.aquatox.2024.106908 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 271 |j 2024 |b 03 |c 04 |h 106908 | ||