Multiscale architecture design of 3D printed biodegradable Zn-based porous scaffolds for immunomodulatory osteogenesis
© 2024. The Author(s)..
Reconciling the dilemma between rapid degradation and overdose toxicity is challenging in biodegradable materials when shifting from bulk to porous materials. Here, we achieve significant bone ingrowth into Zn-based porous scaffolds with 90% porosity via osteoinmunomodulation. At microscale, an alloy incorporating 0.8 wt% Li is employed to create a eutectoid lamellar structure featuring the LiZn4 and Zn phases. This microstructure optimally balances high strength with immunomodulation effects. At mesoscale, surface pattern with nanoscale roughness facilitates filopodia formation and macrophage spreading. At macroscale, the isotropic minimal surface G unit exhibits a proper degradation rate with more uniform feature compared to the anisotropic BCC unit. In vivo, the G scaffold demonstrates a heightened efficiency in promoting macrophage polarization toward an anti-inflammatory phenotype, subsequently leading to significantly elevated osteogenic markers, increased collagen deposition, and enhanced new bone formation. In vitro, transcriptomic analysis reveals the activation of JAK/STAT pathways in macrophages via up regulating the expression of Il-4, Il-10, subsequently promoting osteogenesis.
| Errataetall: |
ErratumIn: Nat Commun. 2024 Apr 29;15(1):3615. - PMID 38684709 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Nature communications - 15(2024), 1 vom: 11. Apr., Seite 3131 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Shuang [VerfasserIn] |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 29.04.2024 published: Electronic ErratumIn: Nat Commun. 2024 Apr 29;15(1):3615. - PMID 38684709 Citation Status MEDLINE |
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| doi: |
10.1038/s41467-024-47189-5 |
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| 520 | |a Reconciling the dilemma between rapid degradation and overdose toxicity is challenging in biodegradable materials when shifting from bulk to porous materials. Here, we achieve significant bone ingrowth into Zn-based porous scaffolds with 90% porosity via osteoinmunomodulation. At microscale, an alloy incorporating 0.8 wt% Li is employed to create a eutectoid lamellar structure featuring the LiZn4 and Zn phases. This microstructure optimally balances high strength with immunomodulation effects. At mesoscale, surface pattern with nanoscale roughness facilitates filopodia formation and macrophage spreading. At macroscale, the isotropic minimal surface G unit exhibits a proper degradation rate with more uniform feature compared to the anisotropic BCC unit. In vivo, the G scaffold demonstrates a heightened efficiency in promoting macrophage polarization toward an anti-inflammatory phenotype, subsequently leading to significantly elevated osteogenic markers, increased collagen deposition, and enhanced new bone formation. In vitro, transcriptomic analysis reveals the activation of JAK/STAT pathways in macrophages via up regulating the expression of Il-4, Il-10, subsequently promoting osteogenesis | ||
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| 700 | 1 | |a Liu, Aobo |e verfasserin |4 aut | |
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| 700 | 1 | |a Huang, He |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Chaoyang |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Jiabao |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Junlong |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Jiahui |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Yan |e verfasserin |4 aut | |
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| 700 | 1 | |a Gu, Xuenan |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaogang |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Yufeng |e verfasserin |4 aut | |
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