Formation of recurring transient Ca2+-based intercellular communities during Drosophila hematopoiesis
Tissue development occurs through a complex interplay between many individual cells. Yet, the fundamental question of how collective tissue behavior emerges from heterogeneous and noisy information processing and transfer at the single-cell level remains unknown. Here, we reveal that tissue scale signaling regulation can arise from local gap-junction mediated cell-cell signaling through the spatiotemporal establishment of an intermediate-scale of transient multicellular communication communities over the course of tissue development. We demonstrated this intermediate scale of emergent signaling using Ca2+ signaling in the intact, ex vivo cultured, live developing Drosophila hematopoietic organ, the lymph gland. Recurrent activation of these transient signaling communities defined self-organized signaling "hotspots" that gradually formed over the course of larva development. These hotspots receive and transmit information to facilitate repetitive interactions with nonhotspot neighbors. Overall, this work bridges the scales between single-cell and emergent group behavior providing key mechanistic insight into how cells establish tissue-scale communication networks.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:121 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 121(2024), 16 vom: 16. Apr., Seite e2318155121 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
David, Saar Ben [VerfasserIn] |
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| Links: |
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| Themen: |
Calcium signaling |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 26.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1073/pnas.2318155121 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM370922425 |
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| 245 | 1 | 0 | |a Formation of recurring transient Ca2+-based intercellular communities during Drosophila hematopoiesis |
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| 520 | |a Tissue development occurs through a complex interplay between many individual cells. Yet, the fundamental question of how collective tissue behavior emerges from heterogeneous and noisy information processing and transfer at the single-cell level remains unknown. Here, we reveal that tissue scale signaling regulation can arise from local gap-junction mediated cell-cell signaling through the spatiotemporal establishment of an intermediate-scale of transient multicellular communication communities over the course of tissue development. We demonstrated this intermediate scale of emergent signaling using Ca2+ signaling in the intact, ex vivo cultured, live developing Drosophila hematopoietic organ, the lymph gland. Recurrent activation of these transient signaling communities defined self-organized signaling "hotspots" that gradually formed over the course of larva development. These hotspots receive and transmit information to facilitate repetitive interactions with nonhotspot neighbors. Overall, this work bridges the scales between single-cell and emergent group behavior providing key mechanistic insight into how cells establish tissue-scale communication networks | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Drosophila hematopoiesis | |
| 650 | 4 | |a calcium signaling | |
| 650 | 4 | |a cell–cell communication | |
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| 700 | 1 | |a Tanentzapf, Guy |e verfasserin |4 aut | |
| 700 | 1 | |a Zaritsky, Assaf |e verfasserin |4 aut | |
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