A long non-coding RNA functions as a competitive endogenous RNA to modulate TaNAC018 by acting as a decoy for tae-miR6206
© 2024. The Author(s), under exclusive licence to Springer Nature B.V..
Increasing evidence indicates a strong correlation between the deposition of cuticular waxes and drought tolerance. However, the precise regulatory mechanism remains elusive. Here, we conducted a comprehensive transcriptome analysis of two wheat (Triticum aestivum) near-isogenic lines, the glaucous line G-JM38 rich in cuticular waxes and the non-glaucous line NG-JM31. We identified 85,143 protein-coding mRNAs, 4,485 lncRNAs, and 1,130 miRNAs. Using the lncRNA-miRNA-mRNA network and endogenous target mimic (eTM) prediction, we discovered that lncRNA35557 acted as an eTM for the miRNA tae-miR6206, effectively preventing tae-miR6206 from cleaving the NAC transcription factor gene TaNAC018. This lncRNA-miRNA interaction led to higher transcript abundance for TaNAC018 and enhanced drought-stress tolerance. Additionally, treatment with mannitol and abscisic acid (ABA) each influenced the levels of tae-miR6206, lncRNA35557, and TaNAC018 transcript. The ectopic expression of TaNAC018 in Arabidopsis also improved tolerance toward mannitol and ABA treatment, whereas knocking down TaNAC018 transcript levels via virus-induced gene silencing in wheat rendered seedlings more sensitive to mannitol stress. Our results indicate that lncRNA35557 functions as a competing endogenous RNA to modulate TaNAC018 expression by acting as a decoy target for tae-miR6206 in glaucous wheat, suggesting that non-coding RNA has important roles in the regulatory mechanisms responsible for wheat stress tolerance.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:114 |
|---|---|
| Enthalten in: |
Plant molecular biology - 114(2024), 3 vom: 10. Apr., Seite 36 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Xu, Wei-Bo [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
3OWL53L36A |
|---|
| Anmerkungen: |
Date Completed 11.04.2024 Date Revised 11.04.2024 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s11103-024-01448-7 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370873467 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370873467 | ||
| 003 | DE-627 | ||
| 005 | 20240411233035.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240410s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11103-024-01448-7 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1372.xml |
| 035 | |a (DE-627)NLM370873467 | ||
| 035 | |a (NLM)38598012 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Xu, Wei-Bo |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A long non-coding RNA functions as a competitive endogenous RNA to modulate TaNAC018 by acting as a decoy for tae-miR6206 |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.04.2024 | ||
| 500 | |a Date Revised 11.04.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024. The Author(s), under exclusive licence to Springer Nature B.V. | ||
| 520 | |a Increasing evidence indicates a strong correlation between the deposition of cuticular waxes and drought tolerance. However, the precise regulatory mechanism remains elusive. Here, we conducted a comprehensive transcriptome analysis of two wheat (Triticum aestivum) near-isogenic lines, the glaucous line G-JM38 rich in cuticular waxes and the non-glaucous line NG-JM31. We identified 85,143 protein-coding mRNAs, 4,485 lncRNAs, and 1,130 miRNAs. Using the lncRNA-miRNA-mRNA network and endogenous target mimic (eTM) prediction, we discovered that lncRNA35557 acted as an eTM for the miRNA tae-miR6206, effectively preventing tae-miR6206 from cleaving the NAC transcription factor gene TaNAC018. This lncRNA-miRNA interaction led to higher transcript abundance for TaNAC018 and enhanced drought-stress tolerance. Additionally, treatment with mannitol and abscisic acid (ABA) each influenced the levels of tae-miR6206, lncRNA35557, and TaNAC018 transcript. The ectopic expression of TaNAC018 in Arabidopsis also improved tolerance toward mannitol and ABA treatment, whereas knocking down TaNAC018 transcript levels via virus-induced gene silencing in wheat rendered seedlings more sensitive to mannitol stress. Our results indicate that lncRNA35557 functions as a competing endogenous RNA to modulate TaNAC018 expression by acting as a decoy target for tae-miR6206 in glaucous wheat, suggesting that non-coding RNA has important roles in the regulatory mechanisms responsible for wheat stress tolerance | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cuticular waxes | |
| 650 | 4 | |a Drought stress | |
| 650 | 4 | |a NAC | |
| 650 | 4 | |a Wheat | |
| 650 | 4 | |a lncRNA | |
| 650 | 4 | |a miRNA | |
| 650 | 7 | |a RNA, Competitive Endogenous |2 NLM | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 650 | 7 | |a Abscisic Acid |2 NLM | |
| 650 | 7 | |a 72S9A8J5GW |2 NLM | |
| 650 | 7 | |a Mannitol |2 NLM | |
| 650 | 7 | |a 3OWL53L36A |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Waxes |2 NLM | |
| 700 | 1 | |a Guo, Qian-Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Dai, Shuang |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Chang-Ai |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Guo-Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Jin-Guang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shi-Zhong |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jian-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Cheng-Chao |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Kang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Plant molecular biology |d 1981 |g 114(2024), 3 vom: 10. Apr., Seite 36 |w (DE-627)NLM012606529 |x 1573-5028 |7 nnns |
| 773 | 1 | 8 | |g volume:114 |g year:2024 |g number:3 |g day:10 |g month:04 |g pages:36 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s11103-024-01448-7 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 114 |j 2024 |e 3 |b 10 |c 04 |h 36 | ||