A role of methionines in the functioning of oxidatively modified fibrinogen
Copyright © 2024 Elsevier B.V. All rights reserved..
Posttranslational modifications in fibrinogen resulting from induced oxidation or oxidative stress in the organism can have deleterious influence on optimal functioning of fibrinogen, causing a disturbance in assembly and properties of fibrin. The protective mechanism supporting the ability of fibrinogen to function in ROS-generating environment remains completely unexplored. The effects of very low and moderately low HOCl/-OCl concentrations on fibrinogen oxidative modifications, the fibrin network structure as well as the kinetics of both fibrinogen-to-fibrin conversion and fibrin hydrolysis have been explored in the current study. As opposed to 25 Μm, HOCl/-OCl, 10 μM HOCl/-OCl did not affect the functional activity of fibrinogen. It is shown for the first time that a number of Met residues, AαMet476, AαMet517, AαMet584, BβMet367, γMet264, and γMet94, identified in 10 μM HOCl/-OCl fibrinogen by the HPLC-MS/MS method, operate as ROS scavengers, performing an important antioxidant function. In turn, this indicates that the fibrinogen structure is adapted to the detrimental action of ROS. The results obtained in our study provide evidence for a protective mechanism responsible for maintaining the structure and functioning of fibrinogen molecules in the bloodstream under conditions of mild and moderate oxidative stress.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1872 |
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| Enthalten in: |
Biochimica et biophysica acta. Proteins and proteomics - 1872(2024), 4 vom: 04. Apr., Seite 141013 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yurina, L V [VerfasserIn] |
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| Links: |
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| Themen: |
Antioxidant methionines |
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| Anmerkungen: |
Date Revised 10.04.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.bbapap.2024.141013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM370717678 |
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| 520 | |a Posttranslational modifications in fibrinogen resulting from induced oxidation or oxidative stress in the organism can have deleterious influence on optimal functioning of fibrinogen, causing a disturbance in assembly and properties of fibrin. The protective mechanism supporting the ability of fibrinogen to function in ROS-generating environment remains completely unexplored. The effects of very low and moderately low HOCl/-OCl concentrations on fibrinogen oxidative modifications, the fibrin network structure as well as the kinetics of both fibrinogen-to-fibrin conversion and fibrin hydrolysis have been explored in the current study. As opposed to 25 Μm, HOCl/-OCl, 10 μM HOCl/-OCl did not affect the functional activity of fibrinogen. It is shown for the first time that a number of Met residues, AαMet476, AαMet517, AαMet584, BβMet367, γMet264, and γMet94, identified in 10 μM HOCl/-OCl fibrinogen by the HPLC-MS/MS method, operate as ROS scavengers, performing an important antioxidant function. In turn, this indicates that the fibrinogen structure is adapted to the detrimental action of ROS. The results obtained in our study provide evidence for a protective mechanism responsible for maintaining the structure and functioning of fibrinogen molecules in the bloodstream under conditions of mild and moderate oxidative stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antioxidant methionines | |
| 650 | 4 | |a Confocal laser scanning microscopy | |
| 650 | 4 | |a Fibrin | |
| 650 | 4 | |a Fibrinogen | |
| 650 | 4 | |a Fibrinolysis | |
| 650 | 4 | |a HPLC-MS/MS | |
| 650 | 4 | |a Hypochlorite-induced oxidation | |
| 650 | 4 | |a Oxidative modifications | |
| 700 | 1 | |a Vasilyeva, A D |e verfasserin |4 aut | |
| 700 | 1 | |a Gavrilina, E S |e verfasserin |4 aut | |
| 700 | 1 | |a Ivanov, V S |e verfasserin |4 aut | |
| 700 | 1 | |a Obydennyi, S I |e verfasserin |4 aut | |
| 700 | 1 | |a Chabin, I A |e verfasserin |4 aut | |
| 700 | 1 | |a Indeykina, M I |e verfasserin |4 aut | |
| 700 | 1 | |a Kononikhin, A S |e verfasserin |4 aut | |
| 700 | 1 | |a Nikolaev, E N |e verfasserin |4 aut | |
| 700 | 1 | |a Rosenfeld, M A |e verfasserin |4 aut | |
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