Engineering Pseudomonas chlororaphis HT66 for the Biosynthesis of Copolymers Containing 3-Hydroxybutyrate and Medium-Chain-Length 3-Hydroxyalkanoates
phaCB was cultured in King's B medium containing 5 g/L glucose and 5 g/L dodecanoic acid. These results demonstrated that P. chlororaphis can be a platform for producing scl-co-mcl PHA and has the potential for industrial application.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
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| Enthalten in: |
Journal of agricultural and food chemistry - 72(2024), 15 vom: 17. Apr., Seite 8684-8692 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deng, Ru-Xiang [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.04.2024 Date Revised 18.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.jafc.4c00777 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM37054062X |
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| 245 | 1 | 0 | |a Engineering Pseudomonas chlororaphis HT66 for the Biosynthesis of Copolymers Containing 3-Hydroxybutyrate and Medium-Chain-Length 3-Hydroxyalkanoates |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Polyhydroxyalkanoates (PHAs) are promising alternatives to petroleum-based plastics, owing to their biodegradability and superior material properties. Here, the controllable biosynthesis of scl-co-mcl PHA containing 3-hydroxybutyrate (3HB) and mcl 3-hydroxyalkanoates was achieved in Pseudomonas chlororaphis HT66. First, key genes involved in fatty acid β-oxidation, the de novo fatty acid biosynthesis pathway, and the phaC1-phaZ-phaC2 operon were deleted to develop a chassis strain. Subsequently, an acetoacetyl-CoA reductase gene phaB and a PHA synthase gene phaC with broad substrate specificity were heterologously expressed for producing and polymerizing the 3HB monomer with mcl 3-hydroxyalkanoates under the assistance of native β-ketothiolase gene phaA. Furthermore, the monomer composition of scl-co-mcl PHA was regulated by adjusting the amount of glucose and dodecanoic acid supplemented. Notably, the cell dry weight and scl-co-mcl PHA content reached 14.2 g/L and 60.1 wt %, respectively, when the engineered strain HT11Δ::phaCB was cultured in King's B medium containing 5 g/L glucose and 5 g/L dodecanoic acid. These results demonstrated that P. chlororaphis can be a platform for producing scl-co-mcl PHA and has the potential for industrial application | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Pseudomonas chlororaphis | |
| 650 | 4 | |a metabolic engineering | |
| 650 | 4 | |a polyhydroxyalkanoate | |
| 650 | 4 | |a synthetic biology | |
| 650 | 4 | |a β-oxidation | |
| 650 | 7 | |a 3-Hydroxybutyric Acid |2 NLM | |
| 650 | 7 | |a TZP1275679 |2 NLM | |
| 650 | 7 | |a Acyltransferases |2 NLM | |
| 650 | 7 | |a EC 2.3.- |2 NLM | |
| 650 | 7 | |a Polyhydroxyalkanoates |2 NLM | |
| 650 | 7 | |a Glucose |2 NLM | |
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| 700 | 1 | |a Li, Hui-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Hong-Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xue-Hong |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:72 |g year:2024 |g number:15 |g day:17 |g month:04 |g pages:8684-8692 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.jafc.4c00777 |3 Volltext |
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