Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues
© 2024 Society of Chemical Industry..
BACKGROUND: The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China.
RESULTS: We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment.
CONCLUSION: In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Pest management science - (2024) vom: 02. Apr. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Li-Feng [VerfasserIn] |
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| Links: |
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| Themen: |
Invasive species |
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| Anmerkungen: |
Date Revised 10.04.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1002/ps.8114 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370530152 |
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| 245 | 1 | 0 | |a Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues |
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| 520 | |a © 2024 Society of Chemical Industry. | ||
| 520 | |a BACKGROUND: The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China | ||
| 520 | |a RESULTS: We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment | ||
| 520 | |a CONCLUSION: In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Phthorimaea absoluta | |
| 650 | 4 | |a invasive species | |
| 650 | 4 | |a loop mediated isothermal amplification (LAMP) | |
| 650 | 4 | |a on‐site diagnostics | |
| 700 | 1 | |a Liu, Ya-Ge |e verfasserin |4 aut | |
| 700 | 1 | |a Tao, Yun-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wan-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jian-Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Chi, Sheng-Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Gui-Fen |e verfasserin |4 aut | |
| 700 | 1 | |a Chu, Dong |e verfasserin |4 aut | |
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