Protein hydrolysates prepared by Alcalase using ultrasound and microwave pretreated almond meal and their characterization
© Association of Food Scientists & Technologists (India) 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law..
The study aimed to optimize ultrasonic (US: 40 kHz/200 W for 10, 20, 30, 40, and 50 min), and microwave (MW: 160 W for 45, 90, 125, 180, and 225 s) pretreatment conditions on protein extraction yield and degree of protein hydrolysis (DH) from almond de-oiled meal, an industrial by-product. First order model was used to describe the kinetics of almond protein hydrolysates obtained with Alcalase. The highest DH, 10.95% was recorded for the US-50 min and 8.87% for MW-45 s; while it was 5.76% for the untreated/control sample. At these optimized pretreatment conditions, a 1.16- and 1.18-fold increment in protein recovery was observed for the US and MW pretreatments, respectively in comparison to the conventional alkaline extraction. The molecular weight distribution recorded for pretreated samples disclosed a significant reduction in the band thickness in comparison with control. Both the pretreatments resulted in a significant increase (P < 0.05) in the antioxidant activity, and TCA solubility index when compared with the control. Results evinced that US and/or MW pretreatments before enzymatic hydrolysis can be a promising approach for the valorization of almond meal for its subsequent use as an ingredient for functional foods/nutraceuticals which otherwise fetches low value as an animal feed.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Journal of food science and technology - 61(2024), 6 vom: 15. Apr., Seite 1157-1164 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sari, T P [VerfasserIn] |
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| Links: |
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| Themen: |
Alcalase hydrolysis |
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| Anmerkungen: |
Date Revised 03.04.2024 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s13197-024-05945-x |
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| Förderinstitution / Projekttitel: |
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| 520 | |a The study aimed to optimize ultrasonic (US: 40 kHz/200 W for 10, 20, 30, 40, and 50 min), and microwave (MW: 160 W for 45, 90, 125, 180, and 225 s) pretreatment conditions on protein extraction yield and degree of protein hydrolysis (DH) from almond de-oiled meal, an industrial by-product. First order model was used to describe the kinetics of almond protein hydrolysates obtained with Alcalase. The highest DH, 10.95% was recorded for the US-50 min and 8.87% for MW-45 s; while it was 5.76% for the untreated/control sample. At these optimized pretreatment conditions, a 1.16- and 1.18-fold increment in protein recovery was observed for the US and MW pretreatments, respectively in comparison to the conventional alkaline extraction. The molecular weight distribution recorded for pretreated samples disclosed a significant reduction in the band thickness in comparison with control. Both the pretreatments resulted in a significant increase (P < 0.05) in the antioxidant activity, and TCA solubility index when compared with the control. Results evinced that US and/or MW pretreatments before enzymatic hydrolysis can be a promising approach for the valorization of almond meal for its subsequent use as an ingredient for functional foods/nutraceuticals which otherwise fetches low value as an animal feed | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Alcalase hydrolysis | |
| 650 | 4 | |a Almond de-oiled residue | |
| 650 | 4 | |a Economics | |
| 650 | 4 | |a First order kinetics | |
| 650 | 4 | |a Microwave pretreatment | |
| 650 | 4 | |a ORAC | |
| 650 | 4 | |a Ultrasonic | |
| 700 | 1 | |a Sirohi, Ranjna |e verfasserin |4 aut | |
| 700 | 1 | |a Tyagi, Prajwal |e verfasserin |4 aut | |
| 700 | 1 | |a Tiwari, Gyanendra |e verfasserin |4 aut | |
| 700 | 1 | |a Pal, Jyotiraditya |e verfasserin |4 aut | |
| 700 | 1 | |a Kunadia, Nihar N |e verfasserin |4 aut | |
| 700 | 1 | |a Verma, Kiran |e verfasserin |4 aut | |
| 700 | 1 | |a Badgujar, Prarabdh C |e verfasserin |4 aut | |
| 700 | 1 | |a Pareek, Sunil |e verfasserin |4 aut | |
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