Serum Prestin After Ototoxin Exposure Is Not Dependent on Outer Hair Cell Loss
Copyright © 2024, Otology & Neurotology, Inc..
HYPOTHESIS: Cyclodextrin (CDX)-induced serum prestin burst is not dependent on outer hair cell (OHC) loss.
BACKGROUND: Serum prestin has been proposed as a biomarker for ototoxicity. We recently used an automated Western approach to quantify serum prestin changes in a newly introduced model of CDX ototoxicity. To gain insights into prestin as a biomarker, here we further characterize serum prestin in the CDX model.
METHODS: Guinea pigs were treated with 750, 3,000, or 4,000 mg/kg CDX, and serum samples were obtained through up to 15 weeks after exposure. Serum prestin levels were quantified using automated Western, and hair cell counts were obtained.
RESULTS: All three doses induced an N-glycosylated ~134-kDa prestin burst; however, only the 3,000 and 4,000 mg/kg resulted in robust OHC loss. Prestin levels returned to baseline where they remained up to 15 weeks in the absence of OHCs.
CONCLUSION: The ~134-kDa prestin burst induced after CDX administration is N-glycosylated, representing a posttranslational modification of prestin. Serum prestin seems to be a promising biomarker when using therapeutics with ototoxic properties because it is not dependent on OHC loss as a necessary event, thus affording the opportunity for early detection and intervention.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology - (2024) vom: 01. Apr. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Harrison, Megan S [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 01.04.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1097/MAO.0000000000004178 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370511034 |
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| 100 | 1 | |a Harrison, Megan S |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Serum Prestin After Ototoxin Exposure Is Not Dependent on Outer Hair Cell Loss |
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| 500 | |a Date Revised 01.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright © 2024, Otology & Neurotology, Inc. | ||
| 520 | |a HYPOTHESIS: Cyclodextrin (CDX)-induced serum prestin burst is not dependent on outer hair cell (OHC) loss | ||
| 520 | |a BACKGROUND: Serum prestin has been proposed as a biomarker for ototoxicity. We recently used an automated Western approach to quantify serum prestin changes in a newly introduced model of CDX ototoxicity. To gain insights into prestin as a biomarker, here we further characterize serum prestin in the CDX model | ||
| 520 | |a METHODS: Guinea pigs were treated with 750, 3,000, or 4,000 mg/kg CDX, and serum samples were obtained through up to 15 weeks after exposure. Serum prestin levels were quantified using automated Western, and hair cell counts were obtained | ||
| 520 | |a RESULTS: All three doses induced an N-glycosylated ~134-kDa prestin burst; however, only the 3,000 and 4,000 mg/kg resulted in robust OHC loss. Prestin levels returned to baseline where they remained up to 15 weeks in the absence of OHCs | ||
| 520 | |a CONCLUSION: The ~134-kDa prestin burst induced after CDX administration is N-glycosylated, representing a posttranslational modification of prestin. Serum prestin seems to be a promising biomarker when using therapeutics with ototoxic properties because it is not dependent on OHC loss as a necessary event, thus affording the opportunity for early detection and intervention | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Driscoll, Brittany G |e verfasserin |4 aut | |
| 700 | 1 | |a Farnsworth, Jason |e verfasserin |4 aut | |
| 700 | 1 | |a Hinton, Ashley |e verfasserin |4 aut | |
| 700 | 1 | |a Peppi, Marcello |e verfasserin |4 aut | |
| 700 | 1 | |a McLean, Will |e verfasserin |4 aut | |
| 700 | 1 | |a Parham, Kourosh |e verfasserin |4 aut | |
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