Metallomic mapping of gut and brain in heavy metal exposed earthworms : A novel paradigm in ecotoxicology
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved..
This study explored the uptake of lead in the epigeic earthworm Dendrobaena veneta exposed to 0, 1000, and 2500 μg Pb/g soil. The soil metal content was extracted using strong acid digestion and water leaching, and analysed by means of Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to estimate absolute and bioavailable concentrations of metals in the soil. The guts and heads of lead-exposed earthworms were processed into formalin-fixed and paraffin embedded sections for high-resolution multi-element metallomic imaging via Laser Ablation ICP-MS (LA-ICP-MS). Metallomic maps of phosphorus, zinc, and lead were produced at 15-μm resolution in the head and gut of D. veneta. Additional 4-μm resolution metallomic maps of the earthworm brains were taken, revealing the detailed localisation of metals in the brain. The Pb bioaccumulated in the chloragogenous tissues of the earthworm in a dose-dependent manner, making it possible to track the extent of soil contamination. The bioaccumulation of P and Zn in earthworm tissues was independent of Pb exposure concentration. This approach demonstrates the utility of LA-ICP-MS as a powerful approach for ecotoxicology and environmental risk assessments.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:709 |
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| Enthalten in: |
Biochemical and biophysical research communications - 709(2024) vom: 21. Apr., Seite 149827 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Karpov, Maxim A [VerfasserIn] |
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| Links: |
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| Themen: |
2P299V784P |
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| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbrc.2024.149827 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a This study explored the uptake of lead in the epigeic earthworm Dendrobaena veneta exposed to 0, 1000, and 2500 μg Pb/g soil. The soil metal content was extracted using strong acid digestion and water leaching, and analysed by means of Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to estimate absolute and bioavailable concentrations of metals in the soil. The guts and heads of lead-exposed earthworms were processed into formalin-fixed and paraffin embedded sections for high-resolution multi-element metallomic imaging via Laser Ablation ICP-MS (LA-ICP-MS). Metallomic maps of phosphorus, zinc, and lead were produced at 15-μm resolution in the head and gut of D. veneta. Additional 4-μm resolution metallomic maps of the earthworm brains were taken, revealing the detailed localisation of metals in the brain. The Pb bioaccumulated in the chloragogenous tissues of the earthworm in a dose-dependent manner, making it possible to track the extent of soil contamination. The bioaccumulation of P and Zn in earthworm tissues was independent of Pb exposure concentration. This approach demonstrates the utility of LA-ICP-MS as a powerful approach for ecotoxicology and environmental risk assessments | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Chloragogenous tissues | |
| 650 | 4 | |a Earthworm | |
| 650 | 4 | |a Ecotoxicology | |
| 650 | 4 | |a LA-ICP-MS | |
| 650 | 4 | |a Lead bioaccumulation | |
| 650 | 4 | |a Soil analysis | |
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| 650 | 7 | |a Soil Pollutants |2 NLM | |
| 700 | 1 | |a Hobbs, Carl |e verfasserin |4 aut | |
| 700 | 1 | |a Jayasinghe, Suwan N |e verfasserin |4 aut | |
| 700 | 1 | |a Stürzenbaum, Stephen R |e verfasserin |4 aut | |
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