Phosphorus deficiency-induced cell wall pectin demethylesterification enhances cadmium accumulation in roots of Salix caprea
Copyright © 2024 Elsevier Ltd. All rights reserved..
Regions affected by heavy metal contamination frequently encounter phosphorus (P) deficiency. Numerous studies highlight crucial role of P in facilitating cadmium (Cd) accumulation in woody plants. However, the regulatory mechanism by which P affects Cd accumulation in roots remains ambiguous. This study aims to investigate the effects of phosphorus (P) deficiency on Cd accumulation, Cd subcellular distribution, and cell wall components in the roots of Salix caprea under Cd stress. The results revealed that under P deficiency conditions, there was a 35.4% elevation in Cd content in roots, coupled with a 60.1% reduction in Cd content in shoots, compared to the P sufficiency conditions. Under deficient P conditions, the predominant response of roots to Cd exposure was the increased sequestration of Cd in root cell walls. The sequestration of Cd in root cell walls increased from 37.1% under sufficient P conditions to 66.7% under P deficiency, with pectin identified as the primary Cd binding site under both P conditions. Among cell wall components, P deficiency led to a significant 31.7% increase in Cd content within pectin compared to P sufficiency conditions, but did not change the pectin content. Notably, P deficiency significantly increased pectin methylesterase (PME) activity by regulating the expression of PME and PMEI genes, leading to a 10.4% reduction in the degree of pectin methylesterification. This may elucidate the absence of significant changes in pectin content under P deficiency conditions and the concurrent increase in Cd accumulation in pectin. Fourier transform infrared spectroscopy (FTIR) results indicated an increase in carboxyl groups in the root cell walls under P deficiency compared to sufficient P treatment. The results provide deep insights into the mechanisms of higher Cd accumulation in root mediated by P deficiency.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:357 |
|---|---|
| Enthalten in: |
Journal of environmental management - 357(2024) vom: 15. Apr., Seite 120691 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Li, Ao [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
00BH33GNGH |
|---|
| Anmerkungen: |
Date Completed 15.04.2024 Date Revised 15.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jenvman.2024.120691 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370439961 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370439961 | ||
| 003 | DE-627 | ||
| 005 | 20240415233615.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240331s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jenvman.2024.120691 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1376.xml |
| 035 | |a (DE-627)NLM370439961 | ||
| 035 | |a (NLM)38554452 | ||
| 035 | |a (PII)S0301-4797(24)00677-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Li, Ao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Phosphorus deficiency-induced cell wall pectin demethylesterification enhances cadmium accumulation in roots of Salix caprea |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 15.04.2024 | ||
| 500 | |a Date Revised 15.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Regions affected by heavy metal contamination frequently encounter phosphorus (P) deficiency. Numerous studies highlight crucial role of P in facilitating cadmium (Cd) accumulation in woody plants. However, the regulatory mechanism by which P affects Cd accumulation in roots remains ambiguous. This study aims to investigate the effects of phosphorus (P) deficiency on Cd accumulation, Cd subcellular distribution, and cell wall components in the roots of Salix caprea under Cd stress. The results revealed that under P deficiency conditions, there was a 35.4% elevation in Cd content in roots, coupled with a 60.1% reduction in Cd content in shoots, compared to the P sufficiency conditions. Under deficient P conditions, the predominant response of roots to Cd exposure was the increased sequestration of Cd in root cell walls. The sequestration of Cd in root cell walls increased from 37.1% under sufficient P conditions to 66.7% under P deficiency, with pectin identified as the primary Cd binding site under both P conditions. Among cell wall components, P deficiency led to a significant 31.7% increase in Cd content within pectin compared to P sufficiency conditions, but did not change the pectin content. Notably, P deficiency significantly increased pectin methylesterase (PME) activity by regulating the expression of PME and PMEI genes, leading to a 10.4% reduction in the degree of pectin methylesterification. This may elucidate the absence of significant changes in pectin content under P deficiency conditions and the concurrent increase in Cd accumulation in pectin. Fourier transform infrared spectroscopy (FTIR) results indicated an increase in carboxyl groups in the root cell walls under P deficiency compared to sufficient P treatment. The results provide deep insights into the mechanisms of higher Cd accumulation in root mediated by P deficiency | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cadmium | |
| 650 | 4 | |a Cell wall | |
| 650 | 4 | |a Pectin methylesterase | |
| 650 | 4 | |a Phosphorus | |
| 650 | 4 | |a Salix caprea | |
| 650 | 7 | |a Pectins |2 NLM | |
| 650 | 7 | |a 89NA02M4RX |2 NLM | |
| 650 | 7 | |a Cadmium |2 NLM | |
| 650 | 7 | |a 00BH33GNGH |2 NLM | |
| 650 | 7 | |a Phosphorus |2 NLM | |
| 650 | 7 | |a 27YLU75U4W |2 NLM | |
| 700 | 1 | |a Wang, Yuancheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Junzhu |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Jiahui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Shaowei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Junxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Zhenyuan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of environmental management |d 2001 |g 357(2024) vom: 15. Apr., Seite 120691 |w (DE-627)NLM112866786 |x 1095-8630 |7 nnns |
| 773 | 1 | 8 | |g volume:357 |g year:2024 |g day:15 |g month:04 |g pages:120691 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jenvman.2024.120691 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 357 |j 2024 |b 15 |c 04 |h 120691 | ||