Bioprospecting of Chlamydomonas reinhardtii for boosting biofuel-related products production based on novel aggregation-induced emission active extracellular polymeric substances nanoprobes
Copyright © 2024 Elsevier Ltd. All rights reserved..
Biofuel production from microalgae has been greatly restricted by low biomass productivity and long-term photosynthetic efficacy. Here, a novel strategy for selecting high-growing, stress-resistant algal strains with high photosynthetic capacity was proposed based on biocompatible extracellular polymeric substances (EPS) probes with aggregation-induced emission (AIE) properties. Specifically, AIE active EPS probes were synthesized for in-situ long-term monitoring of the EPS productivity at different algal growth stages. By coupling the AIE-based fluorescent techniques, algal cells were classified into four diverse populations based on their chlorophyll and EPS signals. Mechanistic studies on the sorted algal cells revealed their remarkable stress resistance and high expression of cell division, biopolymer production and photosynthesis-related genes. The sorted and subcultured algal cells consistently exhibited relatively higher growth rates and photosynthetic capacities, resulting in an increased (1.2 to 1.8-fold) algal biomass production, chlorophyll, and lipids. This study can potentially open new strategies to boost microalgal-based biofuel production.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:399 |
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| Enthalten in: |
Bioresource technology - 399(2024) vom: 26. Apr., Seite 130636 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Yan [VerfasserIn] |
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| Links: |
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| Themen: |
1406-65-1 |
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| Anmerkungen: |
Date Completed 12.04.2024 Date Revised 12.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2024.130636 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Biofuel production from microalgae has been greatly restricted by low biomass productivity and long-term photosynthetic efficacy. Here, a novel strategy for selecting high-growing, stress-resistant algal strains with high photosynthetic capacity was proposed based on biocompatible extracellular polymeric substances (EPS) probes with aggregation-induced emission (AIE) properties. Specifically, AIE active EPS probes were synthesized for in-situ long-term monitoring of the EPS productivity at different algal growth stages. By coupling the AIE-based fluorescent techniques, algal cells were classified into four diverse populations based on their chlorophyll and EPS signals. Mechanistic studies on the sorted algal cells revealed their remarkable stress resistance and high expression of cell division, biopolymer production and photosynthesis-related genes. The sorted and subcultured algal cells consistently exhibited relatively higher growth rates and photosynthetic capacities, resulting in an increased (1.2 to 1.8-fold) algal biomass production, chlorophyll, and lipids. This study can potentially open new strategies to boost microalgal-based biofuel production | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AIE-active EPS probes | |
| 650 | 4 | |a Cell sorting and subculturing | |
| 650 | 4 | |a Chlamydomonas reinhardtii | |
| 650 | 4 | |a Fluorescence-activated cell sorting | |
| 650 | 7 | |a Biofuels |2 NLM | |
| 650 | 7 | |a Chlorophyll |2 NLM | |
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| 700 | 1 | |a Hu, Yubing |e verfasserin |4 aut | |
| 700 | 1 | |a Mo, Jiezhang |e verfasserin |4 aut | |
| 700 | 1 | |a Yan Wong, Tin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Alessandro, Parodi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong Tang, Ben |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wen-Xiong |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Neng |e verfasserin |4 aut | |
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