Dual regulation of cytochrome P450 gene expression by two distinct small RNAs, a novel tasiRNA and miRNA, in Marchantia polymorpha
© The Author(s) 2024. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site–for further information please contact journals.permissions@oup.com..
The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization in the bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded RNA (dsRNA) to generate tasiRNAs, primarily from the 5'- and 3'-ends of dsRNA. Notably, we discovered a novel tasiRNA, tasi78A, can negatively regulate a cytochrome P450 gene, MpCYP78A101. Additionally, tasi78A was abundant in MpAGO1, and transient expression assays underscored the role of tasi78A in repressing MpCYP78A101. A microRNA, miR11700, also regulates MpCYP78A101 expression. This coordinate regulation suggests a role in modulating auxin signaling at apical notches of gemma, influencing the growth and sexual organ development of M. polymorpha and emphasizing the significance of RNA silencing in MpCYP78A101 regulation. However, phylogenetic analysis identified another paralog of the CYP78 family, Mp1g14150, which may have a redundant role with MpCYP78A101, explaining the absence of noticeable morphological changes in loss-of-function plants. Taken together, our findings provide new insights into the combined regulatory roles of miR390/MpTAS3/miR11700 in controlling MpCYP78A101 and expand our knowledge about the biogenesis and regulation of tasiRNAs in M. polymorpha.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Plant & cell physiology - (2024) vom: 28. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hung, Yu-Ling [VerfasserIn] |
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| Links: |
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| Themen: |
Auxin-signaling |
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| Anmerkungen: |
Date Revised 28.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1093/pcp/pcae029 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370352270 |
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| 100 | 1 | |a Hung, Yu-Ling |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Dual regulation of cytochrome P450 gene expression by two distinct small RNAs, a novel tasiRNA and miRNA, in Marchantia polymorpha |
| 264 | 1 | |c 2024 | |
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| 520 | |a © The Author(s) 2024. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site–for further information please contact journals.permissions@oup.com. | ||
| 520 | |a The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization in the bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded RNA (dsRNA) to generate tasiRNAs, primarily from the 5'- and 3'-ends of dsRNA. Notably, we discovered a novel tasiRNA, tasi78A, can negatively regulate a cytochrome P450 gene, MpCYP78A101. Additionally, tasi78A was abundant in MpAGO1, and transient expression assays underscored the role of tasi78A in repressing MpCYP78A101. A microRNA, miR11700, also regulates MpCYP78A101 expression. This coordinate regulation suggests a role in modulating auxin signaling at apical notches of gemma, influencing the growth and sexual organ development of M. polymorpha and emphasizing the significance of RNA silencing in MpCYP78A101 regulation. However, phylogenetic analysis identified another paralog of the CYP78 family, Mp1g14150, which may have a redundant role with MpCYP78A101, explaining the absence of noticeable morphological changes in loss-of-function plants. Taken together, our findings provide new insights into the combined regulatory roles of miR390/MpTAS3/miR11700 in controlling MpCYP78A101 and expand our knowledge about the biogenesis and regulation of tasiRNAs in M. polymorpha | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Marchantia polymorpha | |
| 650 | 4 | |a MpCYP78A101 | |
| 650 | 4 | |a auxin-signaling | |
| 650 | 4 | |a miR11700 | |
| 650 | 4 | |a miR390/MpTAS3 | |
| 650 | 4 | |a tasi78A | |
| 650 | 4 | |a tasiRNA biogenesis | |
| 700 | 1 | |a Hong, Syuan-Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Wei, Wei-Lun |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Shiuan |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Jia-Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Tjita, Veny |e verfasserin |4 aut | |
| 700 | 1 | |a Yong, Qian-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Nishihama, Ryuichi |e verfasserin |4 aut | |
| 700 | 1 | |a Kohchi, Takayuki |e verfasserin |4 aut | |
| 700 | 1 | |a Bowman, John |e verfasserin |4 aut | |
| 700 | 1 | |a Chien, Yuan-Chi |e verfasserin |4 aut | |
| 700 | 1 | |a Chiu, Yen-Hsin |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Ho-Chun |e verfasserin |4 aut | |
| 700 | 1 | |a Jade Lu, Mei-Yeh |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Zhao-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Chun-Neng |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Shih-Shun |e verfasserin |4 aut | |
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