The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods
The interaction between anesthetic Isoflurane (Iso) and model-biomembrane on the water surface has been investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The model-biomembranes used were dipalmitoyl phosphatidyl choline (DPPC), DPPC-palmitic acid (PA) mixture (DPPC:PA = 8:2), DPPC-Alamethicin (Al) mixture (DPPC:Al = 39:1), and DPPC-β-Lactoglobulin (βLG) mixture (DPPC:βLG = 139:1) monolayers, respectively. The quartz crystal oscillator (QCO) was attached horizontally to each monolayer, and QCM and QCI measurements were performed simultaneously. It was found that Iso hydrate physisorbed on each monolayer/water interface from QCM and changed those interfacial viscosities from QCI. With an increase in Iso concentration, pure DPPC, DPPC-PA mixed, and DPPC-Al mixed monolayers showed a two-step process of Iso hydrate on both physisorption and viscosity, whereas it was a one-step for the DPPC-βLG mixed monolayer. The viscosity change in the DPPC-βLG mixed monolayer with the physisorption of Iso hydrate was much larger than that of other monolayers, in spite of the one-step process. From these results, the action mechanism of anesthetics and their relevance to the expression of anesthesia were discussed, based on the "release of interfacial hydrated water" hypothesis on the membrane/water interface.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
Membranes - 14(2024), 3 vom: 27. Feb. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yamamoto, Yasushi [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Revised 29.03.2024 published: Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.3390/membranes14030062 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370248317 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370248317 | ||
| 003 | DE-627 | ||
| 005 | 20240330001706.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240328s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/membranes14030062 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1355.xml |
| 035 | |a (DE-627)NLM370248317 | ||
| 035 | |a (NLM)38535281 | ||
| 035 | |a (PII)62 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Yamamoto, Yasushi |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 29.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a The interaction between anesthetic Isoflurane (Iso) and model-biomembrane on the water surface has been investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The model-biomembranes used were dipalmitoyl phosphatidyl choline (DPPC), DPPC-palmitic acid (PA) mixture (DPPC:PA = 8:2), DPPC-Alamethicin (Al) mixture (DPPC:Al = 39:1), and DPPC-β-Lactoglobulin (βLG) mixture (DPPC:βLG = 139:1) monolayers, respectively. The quartz crystal oscillator (QCO) was attached horizontally to each monolayer, and QCM and QCI measurements were performed simultaneously. It was found that Iso hydrate physisorbed on each monolayer/water interface from QCM and changed those interfacial viscosities from QCI. With an increase in Iso concentration, pure DPPC, DPPC-PA mixed, and DPPC-Al mixed monolayers showed a two-step process of Iso hydrate on both physisorption and viscosity, whereas it was a one-step for the DPPC-βLG mixed monolayer. The viscosity change in the DPPC-βLG mixed monolayer with the physisorption of Iso hydrate was much larger than that of other monolayers, in spite of the one-step process. From these results, the action mechanism of anesthetics and their relevance to the expression of anesthesia were discussed, based on the "release of interfacial hydrated water" hypothesis on the membrane/water interface | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a alamethicin (Al) | |
| 650 | 4 | |a anesthetic hydrate | |
| 650 | 4 | |a dipalmitoyl phosphatidyl choline (DPPC) | |
| 650 | 4 | |a interfacial viscosity | |
| 650 | 4 | |a isoflurane | |
| 650 | 4 | |a mechanism of anesthesia | |
| 650 | 4 | |a monolayer | |
| 650 | 4 | |a palmitic acid (PA) | |
| 650 | 4 | |a physisorption | |
| 650 | 4 | |a quartz crystal impedance (QCI) | |
| 650 | 4 | |a quartz crystal microbalance (QCM) | |
| 650 | 4 | |a simultaneous measurement | |
| 650 | 4 | |a ß-Lactoglobulin (ßLG) | |
| 700 | 1 | |a Ito, Daiki |e verfasserin |4 aut | |
| 700 | 1 | |a Akatsuka, Honoka |e verfasserin |4 aut | |
| 700 | 1 | |a Noguchi, Hiroki |e verfasserin |4 aut | |
| 700 | 1 | |a Matsushita, Arisa |e verfasserin |4 aut | |
| 700 | 1 | |a Kinekawa, Hyuga |e verfasserin |4 aut | |
| 700 | 1 | |a Nagano, Hirotaka |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshino, Akihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Taga, Keijiro |e verfasserin |4 aut | |
| 700 | 1 | |a Shervani, Zameer |e verfasserin |4 aut | |
| 700 | 1 | |a Yamamoto, Masato |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Membranes |d 2010 |g 14(2024), 3 vom: 27. Feb. |w (DE-627)NLM215751760 |x 2077-0375 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2024 |g number:3 |g day:27 |g month:02 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/membranes14030062 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 2024 |e 3 |b 27 |c 02 | ||