Triazole resistance in Aspergillus fumigatus isolated from a tomato production environment exposed to propiconazole
The emergence of azole-resistant Aspergillus fumigatus (ARAf) across the world is an important public health concern. We sought to determine if propiconazole, a demethylase inhibitor (DMI) fungicide, exerted a selective pressure for ARAf in a tomato production environment following multiple exposures to the fungicide. A tomato field trial was established in 2019 and propiconazole was applied weekly until harvest. Soil, leaf, and fruit (when present) samples were collected at baseline and after each propiconazole application. A. fumigatus isolates (n, 178) were recovered and 173 were tested for susceptibility to itraconazole, posaconazole, voriconazole, and propiconazole in accordance with CLSI M38 guidelines. All the isolates were susceptible to medical triazoles and the propiconazole MIC ranged from 0.25 to 8 mg/L. A linear regression model was fitted that showed no longitudinal increment in the log2-fold azole MIC of the isolates collected after each propiconazole exposure compared to the baseline isolates. AsperGenius real-time multiplex assay ruled out TR34/L98H and TR46/Y121F/T289A cyp51A resistance markers in these isolates. Sequencing of a subset of isolates (n, 46) demonstrated widespread presence of F46Y/M172V/E427K and F46Y/M172V/N248T/D255E/E427K cyp51A mutations previously associated with reduced susceptibility to triazoles.
IMPORTANCE: The agricultural use of azole fungicides to control plant diseases has been implicated as a major contributor to ARAf infections in humans. Our study did not reveal imposition of selection pressure for ARAf in a vegetable production system. However, more surveillance studies for ARAf in food crop production and other environments are warranted in understanding this public and One Health issue.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:90 |
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| Enthalten in: |
Applied and environmental microbiology - 90(2024), 4 vom: 17. Apr., Seite e0001724 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
M Jimenez Madrid, Alejandra [VerfasserIn] |
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| Links: |
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| Themen: |
142KW8TBSR |
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| Anmerkungen: |
Date Completed 18.04.2024 Date Revised 25.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/aem.00017-24 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM370236998 |
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| 245 | 1 | 0 | |a Triazole resistance in Aspergillus fumigatus isolated from a tomato production environment exposed to propiconazole |
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| 520 | |a The emergence of azole-resistant Aspergillus fumigatus (ARAf) across the world is an important public health concern. We sought to determine if propiconazole, a demethylase inhibitor (DMI) fungicide, exerted a selective pressure for ARAf in a tomato production environment following multiple exposures to the fungicide. A tomato field trial was established in 2019 and propiconazole was applied weekly until harvest. Soil, leaf, and fruit (when present) samples were collected at baseline and after each propiconazole application. A. fumigatus isolates (n, 178) were recovered and 173 were tested for susceptibility to itraconazole, posaconazole, voriconazole, and propiconazole in accordance with CLSI M38 guidelines. All the isolates were susceptible to medical triazoles and the propiconazole MIC ranged from 0.25 to 8 mg/L. A linear regression model was fitted that showed no longitudinal increment in the log2-fold azole MIC of the isolates collected after each propiconazole exposure compared to the baseline isolates. AsperGenius real-time multiplex assay ruled out TR34/L98H and TR46/Y121F/T289A cyp51A resistance markers in these isolates. Sequencing of a subset of isolates (n, 46) demonstrated widespread presence of F46Y/M172V/E427K and F46Y/M172V/N248T/D255E/E427K cyp51A mutations previously associated with reduced susceptibility to triazoles | ||
| 520 | |a IMPORTANCE: The agricultural use of azole fungicides to control plant diseases has been implicated as a major contributor to ARAf infections in humans. Our study did not reveal imposition of selection pressure for ARAf in a vegetable production system. However, more surveillance studies for ARAf in food crop production and other environments are warranted in understanding this public and One Health issue | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aspergillus fumigatus | |
| 650 | 4 | |a One-Health | |
| 650 | 4 | |a azole-resistance | |
| 650 | 4 | |a azoles | |
| 650 | 4 | |a cyp51A | |
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| 650 | 7 | |a Antifungal Agents |2 NLM | |
| 650 | 7 | |a Fungal Proteins |2 NLM | |
| 650 | 7 | |a Triazoles |2 NLM | |
| 650 | 7 | |a Fungicides, Industrial |2 NLM | |
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| 700 | 1 | |a Rotondo, Francesca |e verfasserin |4 aut | |
| 700 | 1 | |a Deblais, Loic |e verfasserin |4 aut | |
| 700 | 1 | |a Rajashekara, Gireesh |e verfasserin |4 aut | |
| 700 | 1 | |a Miller, Sally A |e verfasserin |4 aut | |
| 700 | 1 | |a Ivey, Melanie L Lewis |e verfasserin |4 aut | |
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