PIV-MyoMonitor : an accessible particle image velocimetry-based software tool for advanced contractility assessment of cardiac organoids
Copyright © 2024 Lee, Kim, Yun, Bae, Park, Jeon, Jang, Lee and Lee..
Induced pluripotent stem cell (iPSC)-derived cardiac organoids offer a versatile platform for personalized cardiac toxicity assessment, drug screening, disease modeling, and regenerative therapies. While previous image-based contractility analysis techniques allowed the assessment of contractility of two-dimensional cardiac models, they face limitations, including encountering high noise levels when applied to three-dimensional organoid models and requiring expensive equipment. Additionally, they offer fewer functional parameters compared to commercial software. To address these challenges, we developed an open-source, particle image velocimetry-based software (PIV-MyoMonitor) and demonstrated its capacity for accurate contractility analysis in both two- and three-dimensional cardiac models using standard lab equipment. Comparisons with four other open-source software programs highlighted the capability of PIV-MyoMonitor for more comprehensive quantitative analysis, providing 22 functional parameters and enhanced video outputs. We showcased its applicability in drug screening by characterizing the response of cardiac organoids to a known isotropic drug, isoprenaline. In sum, PIV-MyoMonitor enables reliable contractility assessment across various cardiac models without costly equipment or software. We believe this software will benefit a broader scientific community.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Frontiers in bioengineering and biotechnology - 12(2024) vom: 21., Seite 1367141 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lee, Hoyeon [VerfasserIn] |
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| Links: |
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| Themen: |
Cardiac models |
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| Anmerkungen: |
Date Revised 28.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3389/fbioe.2024.1367141 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370224248 |
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| 520 | |a Copyright © 2024 Lee, Kim, Yun, Bae, Park, Jeon, Jang, Lee and Lee. | ||
| 520 | |a Induced pluripotent stem cell (iPSC)-derived cardiac organoids offer a versatile platform for personalized cardiac toxicity assessment, drug screening, disease modeling, and regenerative therapies. While previous image-based contractility analysis techniques allowed the assessment of contractility of two-dimensional cardiac models, they face limitations, including encountering high noise levels when applied to three-dimensional organoid models and requiring expensive equipment. Additionally, they offer fewer functional parameters compared to commercial software. To address these challenges, we developed an open-source, particle image velocimetry-based software (PIV-MyoMonitor) and demonstrated its capacity for accurate contractility analysis in both two- and three-dimensional cardiac models using standard lab equipment. Comparisons with four other open-source software programs highlighted the capability of PIV-MyoMonitor for more comprehensive quantitative analysis, providing 22 functional parameters and enhanced video outputs. We showcased its applicability in drug screening by characterizing the response of cardiac organoids to a known isotropic drug, isoprenaline. In sum, PIV-MyoMonitor enables reliable contractility assessment across various cardiac models without costly equipment or software. We believe this software will benefit a broader scientific community | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a IPSC-CM cardiomyocytes | |
| 650 | 4 | |a cardiac models | |
| 650 | 4 | |a contractility | |
| 650 | 4 | |a induced pluripotent stem cells | |
| 650 | 4 | |a organoid | |
| 650 | 4 | |a particle image velocimetry | |
| 700 | 1 | |a Kim, Boyoung |e verfasserin |4 aut | |
| 700 | 1 | |a Yun, Jiyue |e verfasserin |4 aut | |
| 700 | 1 | |a Bae, Jinseung |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Sungsu |e verfasserin |4 aut | |
| 700 | 1 | |a Jeon, Junseok |e verfasserin |4 aut | |
| 700 | 1 | |a Jang, Hye Ryoun |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jaecheol |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Soah |e verfasserin |4 aut | |
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