A c-di-GMP binding effector STM0435 modulates flagellar motility and pathogenicity in Salmonella
Flagella play a crucial role in the invasion process of Salmonella and function as a significant antigen that triggers host pyroptosis. Regulation of flagellar biogenesis is essential for both pathogenicity and immune escape of Salmonella. We identified the conserved and unknown function protein STM0435 as a new flagellar regulator. The ∆stm0435 strain exhibited higher pathogenicity in both cellular and animal infection experiments than the wild-type Salmonella. Proteomic and transcriptomic analyses demonstrated dramatic increases in almost all flagellar genes in the ∆stm0435 strain compared to wild-type Salmonella. In a surface plasmon resonance assay, purified STM0435 protein-bound c-di-GMP had an affinity of ~8.383 µM. The crystal structures of apo-STM0435 and STM0435&c-di-GMP complex were determined. Structural analysis revealed that R33, R137, and D138 of STM0435 were essential for c-di-GMP binding. A Salmonella with STM1987 (GGDEF protein) or STM4264 (EAL protein) overexpression exhibits completely different motility behaviours, indicating that the binding of c-di-GMP to STM0435 promotes its inhibitory effect on Salmonella flagellar biogenesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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| Enthalten in: |
Virulence - 15(2024), 1 vom: 26. März, Seite 2331265 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dai, Yuanji [VerfasserIn] |
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| Links: |
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| Themen: |
61093-23-0 |
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| Anmerkungen: |
Date Completed 28.03.2024 Date Revised 30.03.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1080/21505594.2024.2331265 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370217896 |
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| 245 | 1 | 2 | |a A c-di-GMP binding effector STM0435 modulates flagellar motility and pathogenicity in Salmonella |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Flagella play a crucial role in the invasion process of Salmonella and function as a significant antigen that triggers host pyroptosis. Regulation of flagellar biogenesis is essential for both pathogenicity and immune escape of Salmonella. We identified the conserved and unknown function protein STM0435 as a new flagellar regulator. The ∆stm0435 strain exhibited higher pathogenicity in both cellular and animal infection experiments than the wild-type Salmonella. Proteomic and transcriptomic analyses demonstrated dramatic increases in almost all flagellar genes in the ∆stm0435 strain compared to wild-type Salmonella. In a surface plasmon resonance assay, purified STM0435 protein-bound c-di-GMP had an affinity of ~8.383 µM. The crystal structures of apo-STM0435 and STM0435&c-di-GMP complex were determined. Structural analysis revealed that R33, R137, and D138 of STM0435 were essential for c-di-GMP binding. A Salmonella with STM1987 (GGDEF protein) or STM4264 (EAL protein) overexpression exhibits completely different motility behaviours, indicating that the binding of c-di-GMP to STM0435 promotes its inhibitory effect on Salmonella flagellar biogenesis | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Salmonella typhimurium | |
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| 700 | 1 | |a Liu, Ruirui |e verfasserin |4 aut | |
| 700 | 1 | |a Yue, Yingying |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Nannan |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Haihong |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Zhongrui |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Xueyan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Xilu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Bingqing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Weiwei |e verfasserin |4 aut | |
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