Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis
Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved..
A pH-responsive colorimetric method based on dual-enzyme catalysis for rapid and facile detection and quantification of nanoPET at environment-dependent concentration is proposed. The nanoPET was hydrolyzed by the synergistic catalysis of cutinase and lipase to terephthalic acid which can be sensitive detected using bromocresol purple as the indicator. The color changed from purple to bright yellow as the nanoPET detection concentration increased from 0 mg/mL to 2 mg/mL which can be detected by UV-Vis. This naked-eye method has a high sensitivity for nanoPET detection with the visual detection cutoff of 31.00 μg/mL, and has a good linearity in the range of 0 ∼ 1 mg/mL with LOD of 22.84 μg/mL. The reliability of this method is verified in the detection of nanoPET in lake water and beer samples, with an average recovery of 87.1 %. The as-developed dual-enzyme colorimetric chemosensor holds promising potential as a robust and effective platform for the sensitive detection of nanoPET.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:186 |
|---|---|
| Enthalten in: |
Environment international - 186(2024) vom: 01. Apr., Seite 108598 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhan, Wenchao [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
6S7NKZ40BQ |
|---|
| Anmerkungen: |
Date Completed 24.04.2024 Date Revised 24.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.envint.2024.108598 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM37020784X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM37020784X | ||
| 003 | DE-627 | ||
| 005 | 20240425233316.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240328s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.envint.2024.108598 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1386.xml |
| 035 | |a (DE-627)NLM37020784X | ||
| 035 | |a (NLM)38531236 | ||
| 035 | |a (PII)S0160-4120(24)00184-3 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhan, Wenchao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Naked-eye sensitive detection of nanoPET by pH-responsive colorimetric method based on dual-enzyme catalysis |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.04.2024 | ||
| 500 | |a Date Revised 24.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a A pH-responsive colorimetric method based on dual-enzyme catalysis for rapid and facile detection and quantification of nanoPET at environment-dependent concentration is proposed. The nanoPET was hydrolyzed by the synergistic catalysis of cutinase and lipase to terephthalic acid which can be sensitive detected using bromocresol purple as the indicator. The color changed from purple to bright yellow as the nanoPET detection concentration increased from 0 mg/mL to 2 mg/mL which can be detected by UV-Vis. This naked-eye method has a high sensitivity for nanoPET detection with the visual detection cutoff of 31.00 μg/mL, and has a good linearity in the range of 0 ∼ 1 mg/mL with LOD of 22.84 μg/mL. The reliability of this method is verified in the detection of nanoPET in lake water and beer samples, with an average recovery of 87.1 %. The as-developed dual-enzyme colorimetric chemosensor holds promising potential as a robust and effective platform for the sensitive detection of nanoPET | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ELISA | |
| 650 | 4 | |a Enzyme catalysis | |
| 650 | 4 | |a Naked eye | |
| 650 | 4 | |a Nanoplastic | |
| 650 | 4 | |a Rapid detection | |
| 650 | 7 | |a Phthalic Acids |2 NLM | |
| 650 | 7 | |a Lipase |2 NLM | |
| 650 | 7 | |a EC 3.1.1.3 |2 NLM | |
| 650 | 7 | |a terephthalic acid |2 NLM | |
| 650 | 7 | |a 6S7NKZ40BQ |2 NLM | |
| 700 | 1 | |a Wang, Chenxu |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiyu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hongtao |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Sicheng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xiaoyang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Environment international |d 1994 |g 186(2024) vom: 01. Apr., Seite 108598 |w (DE-627)NLM077828615 |x 1873-6750 |7 nnns |
| 773 | 1 | 8 | |g volume:186 |g year:2024 |g day:01 |g month:04 |g pages:108598 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.envint.2024.108598 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 186 |j 2024 |b 01 |c 04 |h 108598 | ||