The GARP family transcription factor MtHHO3 negatively regulates salt tolerance in Medicago truncatula
Copyright © 2024 Elsevier Masson SAS. All rights reserved..
High salinity is one of the detrimental environmental factors restricting plant growth and crop production throughout the world. This study demonstrated that the GARP family transcription factor MtHHO3 is involved in response to salt stress and abscisic acid (ABA) signaling in Medicago truncatula. The transcription of MtHHO3 was repressed by salt, osmotic stress, and ABA treatment. The seed germination assay showed that, overexpression of MtHHO3 in Arabidopsis thaliana caused hypersensitivity to salt and osmotic stress, but increased resistance to ABA inhibition. Overexpression of MtHHO3 in M. truncatula resulted in decreased tolerance of salinity, while loss-of-function mutants mthho3-1 and mthho3-2 were more resistant to salt stress compared with wild-type plants. qRT-PCR analyses showed that MtHHO3 downregulated the expression of genes in stress and ABA responsive pathways. We further demonstrated that MtHHO3 repressed the transcription of the pathogenesis-related gene MtPR2 by binding to its promoter. Overall, these results indicate that MtHHO3 negatively regulates salt stress response in plants and deepen our understanding of the role of the GARP subfamily transcription factors in modulating salt stress and ABA signaling.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:209 |
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| Enthalten in: |
Plant physiology and biochemistry : PPB - 209(2024) vom: 26. Apr., Seite 108542 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Xue [VerfasserIn] |
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| Links: |
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| Themen: |
72S9A8J5GW |
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| Anmerkungen: |
Date Completed 08.04.2024 Date Revised 08.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.plaphy.2024.108542 |
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| 245 | 1 | 4 | |a The GARP family transcription factor MtHHO3 negatively regulates salt tolerance in Medicago truncatula |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Masson SAS. All rights reserved. | ||
| 520 | |a High salinity is one of the detrimental environmental factors restricting plant growth and crop production throughout the world. This study demonstrated that the GARP family transcription factor MtHHO3 is involved in response to salt stress and abscisic acid (ABA) signaling in Medicago truncatula. The transcription of MtHHO3 was repressed by salt, osmotic stress, and ABA treatment. The seed germination assay showed that, overexpression of MtHHO3 in Arabidopsis thaliana caused hypersensitivity to salt and osmotic stress, but increased resistance to ABA inhibition. Overexpression of MtHHO3 in M. truncatula resulted in decreased tolerance of salinity, while loss-of-function mutants mthho3-1 and mthho3-2 were more resistant to salt stress compared with wild-type plants. qRT-PCR analyses showed that MtHHO3 downregulated the expression of genes in stress and ABA responsive pathways. We further demonstrated that MtHHO3 repressed the transcription of the pathogenesis-related gene MtPR2 by binding to its promoter. Overall, these results indicate that MtHHO3 negatively regulates salt stress response in plants and deepen our understanding of the role of the GARP subfamily transcription factors in modulating salt stress and ABA signaling | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a ABA signaling | |
| 650 | 4 | |a GARP | |
| 650 | 4 | |a MtHHO3 | |
| 650 | 4 | |a Salt stress | |
| 650 | 4 | |a Transcription factor | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a Abscisic Acid |2 NLM | |
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| 700 | 1 | |a Wei, Chunxue |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Hongmei |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Junmei |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Ruicai |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Lin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Mingna |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Qingchuan |e verfasserin |4 aut | |
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