Mutations influence the conformational dynamics of the GDP/KRAS complex
Mutations near allosteric sites can have a significant impact on the function of KRAS. Three specific mutations, K104Q, G12D/K104Q, and G12D/G75A, which are located near allosteric positions, were selected to investigate the molecular mechanisms behind mutation-induced influences on the activity of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations followed by the principal component analysis (PCA) were performed to improve the sampling of conformational states. The results revealed that these mutations significantly alter the structural flexibility, correlated motions, and dynamic behavior of the switch regions that are essential for KRAS binding to effectors or regulators. Furthermore, the mutations have a significant impact on the hydrogen bonding interactions between GDP and the switch regions, as well as on the electrostatic interactions of magnesium ions (Mg2+) with these regions. Our results verified that these mutations strongly influence the binding of KRAS to its effectors or regulators and allosterically regulate the activity. We believe that this work can provide valuable theoretical insights into a deeper understanding of KRAS function.Communicated by Ramaswamy H. Sarma.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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Enthalten in: |
Journal of biomolecular structure & dynamics - (2024) vom: 26. März, Seite 1-14 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Shen, Congcong [VerfasserIn] |
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Links: |
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Themen: |
Conformational dynamics |
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Anmerkungen: |
Date Revised 26.03.2024 published: Print-Electronic Citation Status Publisher |
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doi: |
10.1080/07391102.2024.2331627 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM370194802 |
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520 | |a Mutations near allosteric sites can have a significant impact on the function of KRAS. Three specific mutations, K104Q, G12D/K104Q, and G12D/G75A, which are located near allosteric positions, were selected to investigate the molecular mechanisms behind mutation-induced influences on the activity of KRAS. Gaussian accelerated molecular dynamics (GaMD) simulations followed by the principal component analysis (PCA) were performed to improve the sampling of conformational states. The results revealed that these mutations significantly alter the structural flexibility, correlated motions, and dynamic behavior of the switch regions that are essential for KRAS binding to effectors or regulators. Furthermore, the mutations have a significant impact on the hydrogen bonding interactions between GDP and the switch regions, as well as on the electrostatic interactions of magnesium ions (Mg2+) with these regions. Our results verified that these mutations strongly influence the binding of KRAS to its effectors or regulators and allosterically regulate the activity. We believe that this work can provide valuable theoretical insights into a deeper understanding of KRAS function.Communicated by Ramaswamy H. Sarma | ||
650 | 4 | |a Journal Article | |
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700 | 1 | |a Wang, Min |e verfasserin |4 aut | |
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700 | 1 | |a Xu, Xin |e verfasserin |4 aut | |
700 | 1 | |a Zhou, Zhongshun |e verfasserin |4 aut | |
700 | 1 | |a Hu, Yingshi |e verfasserin |4 aut | |
700 | 1 | |a Xia, Caijuan |e verfasserin |4 aut | |
700 | 1 | |a Hu, Guodong |e verfasserin |4 aut | |
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