Generation of novel anti-apoE monoclonal antibodies that selectively recognize apoE isoforms
© 2024 Federation of European Biochemical Societies..
Apolipoprotein E (apoE) is a regulator of lipid metabolism, cholesterol transport, and the clearance and aggregation of amyloid β in the brain. The three human apoE isoforms apoE2, apoE3, and apoE4 only differ in one or two residues. Nevertheless, the functions highly depend on the isoform types and lipidated states. Here, we generated novel anti-apoE monoclonal antibodies (mAbs) and obtained an apoE4-selective mAb whose epitope is within residues 110-117. ELISA and bio-layer interferometry measurements demonstrated that the dissociation constants of mAbs are within the nanomolar range. Using the generated antibodies, we successfully constructed sandwich ELISA systems, which can detect all apoE isoforms or selectively detect apoE4. These results suggest the usability of the generated anti-apoE mAbs for selective detection of apoE isoforms.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:598 |
|---|---|
| Enthalten in: |
FEBS letters - 598(2024), 8 vom: 22. Apr., Seite 902-914 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ohgita, Takashi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antibodies, Monoclonal |
|---|
| Anmerkungen: |
Date Completed 23.04.2024 Date Revised 24.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/1873-3468.14858 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370192613 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370192613 | ||
| 003 | DE-627 | ||
| 005 | 20240424232057.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240327s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/1873-3468.14858 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1385.xml |
| 035 | |a (DE-627)NLM370192613 | ||
| 035 | |a (NLM)38529702 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ohgita, Takashi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Generation of novel anti-apoE monoclonal antibodies that selectively recognize apoE isoforms |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.04.2024 | ||
| 500 | |a Date Revised 24.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024 Federation of European Biochemical Societies. | ||
| 520 | |a Apolipoprotein E (apoE) is a regulator of lipid metabolism, cholesterol transport, and the clearance and aggregation of amyloid β in the brain. The three human apoE isoforms apoE2, apoE3, and apoE4 only differ in one or two residues. Nevertheless, the functions highly depend on the isoform types and lipidated states. Here, we generated novel anti-apoE monoclonal antibodies (mAbs) and obtained an apoE4-selective mAb whose epitope is within residues 110-117. ELISA and bio-layer interferometry measurements demonstrated that the dissociation constants of mAbs are within the nanomolar range. Using the generated antibodies, we successfully constructed sandwich ELISA systems, which can detect all apoE isoforms or selectively detect apoE4. These results suggest the usability of the generated anti-apoE mAbs for selective detection of apoE isoforms | ||
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a apoE isoform | |
| 650 | 4 | |a monoclonal antibody | |
| 650 | 4 | |a sandwich ELISA | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Protein Isoforms |2 NLM | |
| 650 | 7 | |a Apolipoproteins E |2 NLM | |
| 650 | 7 | |a Epitopes |2 NLM | |
| 650 | 7 | |a Apolipoprotein E4 |2 NLM | |
| 650 | 7 | |a Apolipoprotein E3 |2 NLM | |
| 700 | 1 | |a Sakai, Koto |e verfasserin |4 aut | |
| 700 | 1 | |a Fukui, Nodoka |e verfasserin |4 aut | |
| 700 | 1 | |a Namba, Norihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nakano, Miyu |e verfasserin |4 aut | |
| 700 | 1 | |a Kiguchi, Yuki |e verfasserin |4 aut | |
| 700 | 1 | |a Morita, Izumi |e verfasserin |4 aut | |
| 700 | 1 | |a Oyama, Hiroyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaki, Kouya |e verfasserin |4 aut | |
| 700 | 1 | |a Nagao, Kohjiro |e verfasserin |4 aut | |
| 700 | 1 | |a Kobayashi, Norihiro |e verfasserin |4 aut | |
| 700 | 1 | |a Saito, Hiroyuki |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t FEBS letters |d 1968 |g 598(2024), 8 vom: 22. Apr., Seite 902-914 |w (DE-627)NLM000016691 |x 1873-3468 |7 nnns |
| 773 | 1 | 8 | |g volume:598 |g year:2024 |g number:8 |g day:22 |g month:04 |g pages:902-914 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/1873-3468.14858 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 598 |j 2024 |e 8 |b 22 |c 04 |h 902-914 | ||