Identification and Functional Characterization of an Omega-Class Glutathione S-Transferase Gene PcGSTO1 Associated with Cyetpyrafen Resistance in Panonychus citri (McGregor)

Cyetpyrafen is a recently developed acaricide. The citrus red mite, Panonychus citri (McGregor), has developed significant resistance to cyetpyrafen. However, the molecular mechanism underlying the cyetpyrafen resistance in P. citri remains unclear. Glutathione S-transferases (GSTs) play a critical role in arthropod pesticide resistance. This study showed that GSTs were potentially related to the resistance of P. citri to cyetpyrafen through synergistic experiments and enzyme activity analysis. An omega-family GST gene, PcGSTO1, was significantly up-regulated in the egg, nymph, and adult stages of the cyetpyrafen-resistant strain. Additionally, silencing of PcGSTO1 significantly increased the mortality of P. citri to cyetpyrafen and recombinant PcGSTO1 demonstrated the ability to metabolize cyetpyrafen. Our results indicated that the overexpression of PcGSTO1 is associated with cyetpyrafen resistance in P. citri, and they also provided valuable information for managing resistance in P. citri.

Medienart:

E-Artikel

Erscheinungsjahr:

2024

Erschienen:

2024

Enthalten in:

Zur Gesamtaufnahme - volume:72

Enthalten in:

Journal of agricultural and food chemistry - 72(2024), 13 vom: 03. Apr., Seite 7010-7020

Sprache:

Englisch

Beteiligte Personen:

Ding, Li-Li [VerfasserIn]
Yu, Shi-Jiang [VerfasserIn]
Lei, Shuang [VerfasserIn]
Pan, Qi [VerfasserIn]
Liu, Liu [VerfasserIn]
Li, Si-Chen [VerfasserIn]
Chen, Ting-Yu [VerfasserIn]
Wang, Shu-Qi [VerfasserIn]
Wei, Zhi-Tang [VerfasserIn]
Liu, Hao-Qiang [VerfasserIn]
Cong, Lin [VerfasserIn]
Ran, Chun [VerfasserIn]

Links:

Volltext

Themen:

Acaricides
Cyetpyrafen resistance
EC 2.5.1.18
Glutathione Transferase
Glutathione-s-transferase
Journal Article
Metabolism
Panonychus citri

Anmerkungen:

Date Completed 04.04.2024

Date Revised 04.04.2024

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acs.jafc.4c00732

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM370190912