Transcriptomic insights into the lipotoxicity of high-fat high-fructose diet in rat and mouse
Copyright © 2024 Elsevier Inc. All rights reserved..
Along with the increasing prevalence of obesity worldwide, the deleterious effects of high-calorie diet are gradually recognized through more and more epidemiological studies. However, the concealed and chronic causality whitewashes its unhealthy character. Given an ingenious mechanism orchestrates the metabolic adaptation to high-fat high-fructose (HFF) diet and connive its lipotoxicity, in this study, an experimental rat/mouse model of obesity was induced and a comparative transcriptomic analysis was performed to probe the mystery. Our results demonstrated that HFF diet consumption altered the transcriptomic pattern as well as different high-calorie diet fed rat/mouse manifested distinct hepatic transcriptome. Validation with RT-qPCR and Western blotting confirmed that SREBP1-FASN involved in de novo lipogenesis partly mediated metabolic self-adaption. Moreover, hepatic ACSL1-CPT1A-CPT2 pathway involved in fatty acids β-oxidation, played a key role in the metabolic adaption to HFF. Collectively, our findings enrich the knowledge of the chronic adaptation mechanisms and also shed light on future investigations. Meanwhile, our results also suggest that efforts to restore the fatty acids metabolic fate could be a promising avenue to fight against obesity and associated steatosis and insulin resistance challenged by HFF diet.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:128 |
|---|---|
| Enthalten in: |
The Journal of nutritional biochemistry - 128(2024) vom: 03. Apr., Seite 109626 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhu, An-Qi [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 27.04.2024 Date Revised 27.04.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jnutbio.2024.109626 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370171217 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370171217 | ||
| 003 | DE-627 | ||
| 005 | 20240428231958.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240327s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jnutbio.2024.109626 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1391.xml |
| 035 | |a (DE-627)NLM370171217 | ||
| 035 | |a (NLM)38527560 | ||
| 035 | |a (PII)S0955-2863(24)00059-7 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhu, An-Qi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Transcriptomic insights into the lipotoxicity of high-fat high-fructose diet in rat and mouse |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 27.04.2024 | ||
| 500 | |a Date Revised 27.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2024 Elsevier Inc. All rights reserved. | ||
| 520 | |a Along with the increasing prevalence of obesity worldwide, the deleterious effects of high-calorie diet are gradually recognized through more and more epidemiological studies. However, the concealed and chronic causality whitewashes its unhealthy character. Given an ingenious mechanism orchestrates the metabolic adaptation to high-fat high-fructose (HFF) diet and connive its lipotoxicity, in this study, an experimental rat/mouse model of obesity was induced and a comparative transcriptomic analysis was performed to probe the mystery. Our results demonstrated that HFF diet consumption altered the transcriptomic pattern as well as different high-calorie diet fed rat/mouse manifested distinct hepatic transcriptome. Validation with RT-qPCR and Western blotting confirmed that SREBP1-FASN involved in de novo lipogenesis partly mediated metabolic self-adaption. Moreover, hepatic ACSL1-CPT1A-CPT2 pathway involved in fatty acids β-oxidation, played a key role in the metabolic adaption to HFF. Collectively, our findings enrich the knowledge of the chronic adaptation mechanisms and also shed light on future investigations. Meanwhile, our results also suggest that efforts to restore the fatty acids metabolic fate could be a promising avenue to fight against obesity and associated steatosis and insulin resistance challenged by HFF diet | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a ACSL1-CPT1A-CPT2 pathway | |
| 650 | 4 | |a Comparative transcriptomics analysis | |
| 650 | 4 | |a High-fat high-fructose diet | |
| 650 | 4 | |a de novo lipogenesis | |
| 650 | 7 | |a Fructose |2 NLM | |
| 650 | 7 | |a 30237-26-4 |2 NLM | |
| 650 | 7 | |a Sterol Regulatory Element Binding Protein 1 |2 NLM | |
| 650 | 7 | |a Srebf1 protein, rat |2 NLM | |
| 650 | 7 | |a FASN protein, rat |2 NLM | |
| 650 | 7 | |a EC 2.3.1.85 |2 NLM | |
| 650 | 7 | |a Fasn protein, mouse |2 NLM | |
| 650 | 7 | |a EC 2.3.1.85 |2 NLM | |
| 650 | 7 | |a Fatty Acids |2 NLM | |
| 650 | 7 | |a Fatty Acid Synthase, Type I |2 NLM | |
| 650 | 7 | |a EC 2.3.1.85 |2 NLM | |
| 700 | 1 | |a Luo, Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xiao-Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Chu-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Tian-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Kun-Ping |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The Journal of nutritional biochemistry |d 1990 |g 128(2024) vom: 03. Apr., Seite 109626 |w (DE-627)NLM089754956 |x 1873-4847 |7 nnns |
| 773 | 1 | 8 | |g volume:128 |g year:2024 |g day:03 |g month:04 |g pages:109626 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jnutbio.2024.109626 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 128 |j 2024 |b 03 |c 04 |h 109626 | ||