Single cell analyses of cancer cells identified two regulatorily and functionally distinct categories in differentially expressed genes among tumor subclones
© 2024 The Authors..
To explore the feature of cancer cells and tumor subclones, we analyzed 101,065 single-cell transcriptomes from 12 colorectal cancer (CRC) patients and 92 single cell genomes from one of these patients. We found cancer cells, endothelial cells and stromal cells in tumor tissue expressed much more genes and had stronger cell-cell interactions than their counterparts in normal tissue. We identified copy number variations (CNVs) in each cancer cell and found correlation between gene copy number and expression level in cancer cells at single cell resolution. Analysis of tumor subclones inferred by CNVs showed accumulation of mutations in each tumor subclone along lineage trajectories. We found differentially expressed genes (DEGs) between tumor subclones had two populations: DEGCNV and DEGreg. DEGCNV, showing high CNV-expression correlation and whose expression differences depend on the differences of CNV level, enriched in housekeeping genes and cell adhesion associated genes. DEGreg, showing low CNV-expression correlation and mainly in low CNV variation regions and regions without CNVs, enriched in cytokine signaling genes. Furthermore, cell-cell communication analyses showed that DEGCNV tends to involve in cell-cell contact while DEGreg tends to involve in secreted signaling, which further support that DEGCNV and DEGreg are two regulatorily and functionally distinct categories.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2024 |
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Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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Enthalten in: |
Heliyon - 10(2024), 6 vom: 30. März, Seite e28071 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Cao, Wei [VerfasserIn] |
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Links: |
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Themen: |
Cell-cell communication |
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Anmerkungen: |
Date Revised 26.03.2024 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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doi: |
10.1016/j.heliyon.2024.e28071 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM370141717 |
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520 | |a To explore the feature of cancer cells and tumor subclones, we analyzed 101,065 single-cell transcriptomes from 12 colorectal cancer (CRC) patients and 92 single cell genomes from one of these patients. We found cancer cells, endothelial cells and stromal cells in tumor tissue expressed much more genes and had stronger cell-cell interactions than their counterparts in normal tissue. We identified copy number variations (CNVs) in each cancer cell and found correlation between gene copy number and expression level in cancer cells at single cell resolution. Analysis of tumor subclones inferred by CNVs showed accumulation of mutations in each tumor subclone along lineage trajectories. We found differentially expressed genes (DEGs) between tumor subclones had two populations: DEGCNV and DEGreg. DEGCNV, showing high CNV-expression correlation and whose expression differences depend on the differences of CNV level, enriched in housekeeping genes and cell adhesion associated genes. DEGreg, showing low CNV-expression correlation and mainly in low CNV variation regions and regions without CNVs, enriched in cytokine signaling genes. Furthermore, cell-cell communication analyses showed that DEGCNV tends to involve in cell-cell contact while DEGreg tends to involve in secreted signaling, which further support that DEGCNV and DEGreg are two regulatorily and functionally distinct categories | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Cell-cell communication | |
650 | 4 | |a Colorectal cancer | |
650 | 4 | |a Copy number variations | |
650 | 4 | |a Differentially expressed gene | |
650 | 4 | |a Single cell RNA sequencing | |
650 | 4 | |a Single cell whole genome sequencing | |
700 | 1 | |a Wang, Xuefei |e verfasserin |4 aut | |
700 | 1 | |a Luo, Kaiwen |e verfasserin |4 aut | |
700 | 1 | |a Li, Yang |e verfasserin |4 aut | |
700 | 1 | |a Sun, Jiahong |e verfasserin |4 aut | |
700 | 1 | |a Fu, Ruqing |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Qi |e verfasserin |4 aut | |
700 | 1 | |a Hong, Ni |e verfasserin |4 aut | |
700 | 1 | |a Cheung, Edwin |e verfasserin |4 aut | |
700 | 1 | |a Jin, Wenfei |e verfasserin |4 aut | |
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