Rapid identification,fluconazole and micafungin susceptibility testing of Candida species from blood culture by a short incubation method
Copyright © 2024 Elsevier Inc. All rights reserved..
This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92.05 %. Regarding the rapid broth microdilution for Fluconazole and Micafungin, the agreement was 97.06 % (p<0.001) and 100 % (p<0.001), and the Kappa coefficient was 0.91 (p<0.001) and 1.0 (p<0.001), respectively. To conclude, both rapid methods showed to be reproducible, inexpensive, easy to perform and time-saving. Thus, these methodologies could be useful to guide and adjust empirical antifungal therapy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:109 |
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| Enthalten in: |
Diagnostic microbiology and infectious disease - 109(2024), 2 vom: 19. März, Seite 116271 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
de Souza, Ândrea Celestino [VerfasserIn] |
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| Links: |
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| Themen: |
Blood culture |
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| Anmerkungen: |
Date Revised 24.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1016/j.diagmicrobio.2024.116271 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370119347 |
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| 520 | |a This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92.05 %. Regarding the rapid broth microdilution for Fluconazole and Micafungin, the agreement was 97.06 % (p<0.001) and 100 % (p<0.001), and the Kappa coefficient was 0.91 (p<0.001) and 1.0 (p<0.001), respectively. To conclude, both rapid methods showed to be reproducible, inexpensive, easy to perform and time-saving. Thus, these methodologies could be useful to guide and adjust empirical antifungal therapy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Blood culture | |
| 650 | 4 | |a Candida | |
| 650 | 4 | |a MALDI-TOF MS | |
| 650 | 4 | |a Microdilution | |
| 650 | 4 | |a Rapid diagnosis | |
| 700 | 1 | |a Pereira, Dariane Castro |e verfasserin |4 aut | |
| 700 | 1 | |a Barth, Patricia Orlandi |e verfasserin |4 aut | |
| 700 | 1 | |a Roesch, Eliane Würdig |e verfasserin |4 aut | |
| 700 | 1 | |a Lutz, Larissa |e verfasserin |4 aut | |
| 700 | 1 | |a Aquino, Valério Rodrigues |e verfasserin |4 aut | |
| 700 | 1 | |a Goldani, Luciano Zubaran |e verfasserin |4 aut | |
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