Development of a stable genetic transformation system in Erigeron breviscapus : a case study with EbYUC2 in relation to leaf number and flowering time
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature..
MAIN CONCLUSION: A stable genetic transformation system for Erigeron breviscapus was developed. We cloned the EbYUC2 gene and genetically transformed it into Arabidopsis thaliana and E. breviscapus. The leaf number, YUC2 gene expression, and the endogenous auxin content in transgenic plants were significantly increased. Erigeron breviscapus is a prescription drug for the clinical treatment of cardiovascular and cerebrovascular diseases. The rosette leaves have the highest content of the major active compound scutellarin and are an important component in the yield of E. breviscapus. However, little is known about the genes related to the leaf number and flowering time of E. breviscapus. In our previous study, we identified three candidate genes related to the leaf number and flowering of E. breviscapus by combining resequencing data and genome-wide association study (GWAS). However, their specific functions remain to be characterized. In this study, we cloned and transformed the previously identified full-length EbYUC2 gene into Arabidopsis thaliana, developed the first stable genetic transformation system for E. breviscapus, and obtained the transgenic plants overexpressing EbYUC2. Compared with wild-type plants, the transgenic plants showed a significant increase in the number of leaves, which was correlated with the increased expression of EbYUC2. Consistently, the endogenous auxin content, particularly indole-3-acetic acid, in transgenic plants was also significantly increased. These results suggest that EbYUC2 may control the leaf number by regulating auxin biosynthesis, thereby laying a foundation for revealing the molecular mechanism governing the leaf number and flowering time of E. breviscapus.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:259 |
|---|---|
| Enthalten in: |
Planta - 259(2024), 5 vom: 24. März, Seite 98 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhu, Qin [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Auxin |
|---|
| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 23.04.2024 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00425-024-04351-z |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370116097 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370116097 | ||
| 003 | DE-627 | ||
| 005 | 20240423232239.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240324s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00425-024-04351-z |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1384.xml |
| 035 | |a (DE-627)NLM370116097 | ||
| 035 | |a (NLM)38522041 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhu, Qin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Development of a stable genetic transformation system in Erigeron breviscapus |b a case study with EbYUC2 in relation to leaf number and flowering time |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.03.2024 | ||
| 500 | |a Date Revised 23.04.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. | ||
| 520 | |a MAIN CONCLUSION: A stable genetic transformation system for Erigeron breviscapus was developed. We cloned the EbYUC2 gene and genetically transformed it into Arabidopsis thaliana and E. breviscapus. The leaf number, YUC2 gene expression, and the endogenous auxin content in transgenic plants were significantly increased. Erigeron breviscapus is a prescription drug for the clinical treatment of cardiovascular and cerebrovascular diseases. The rosette leaves have the highest content of the major active compound scutellarin and are an important component in the yield of E. breviscapus. However, little is known about the genes related to the leaf number and flowering time of E. breviscapus. In our previous study, we identified three candidate genes related to the leaf number and flowering of E. breviscapus by combining resequencing data and genome-wide association study (GWAS). However, their specific functions remain to be characterized. In this study, we cloned and transformed the previously identified full-length EbYUC2 gene into Arabidopsis thaliana, developed the first stable genetic transformation system for E. breviscapus, and obtained the transgenic plants overexpressing EbYUC2. Compared with wild-type plants, the transgenic plants showed a significant increase in the number of leaves, which was correlated with the increased expression of EbYUC2. Consistently, the endogenous auxin content, particularly indole-3-acetic acid, in transgenic plants was also significantly increased. These results suggest that EbYUC2 may control the leaf number by regulating auxin biosynthesis, thereby laying a foundation for revealing the molecular mechanism governing the leaf number and flowering time of E. breviscapus | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a EbYUC2 | |
| 650 | 4 | |a Erigeron breviscapus | |
| 650 | 4 | |a Auxin | |
| 650 | 4 | |a Genetic transformation | |
| 650 | 4 | |a Leaf number | |
| 650 | 7 | |a Indoleacetic Acids |2 NLM | |
| 700 | 1 | |a Lu, Ying-Chun |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Jing-Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yun-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jian-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Sheng-Chao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Guang-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Sha, Ben-Cai |e verfasserin |4 aut | |
| 700 | 1 | |a He, Si-Mei |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Planta |d 1966 |g 259(2024), 5 vom: 24. März, Seite 98 |w (DE-627)NLM012307033 |x 1432-2048 |7 nnns |
| 773 | 1 | 8 | |g volume:259 |g year:2024 |g number:5 |g day:24 |g month:03 |g pages:98 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00425-024-04351-z |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 259 |j 2024 |e 5 |b 24 |c 03 |h 98 | ||