Quantitative and structural changes of blood platelet cytoskeleton proteins in multiple sclerosis (MS)
Copyright © 2024. Published by Elsevier Ltd..
Epidemiological studies show that cardiovascular events related to platelet hyperactivity remain the leading causes of death among multiple sclerosis (MS) patients. Quantitative or structural changes of platelet cytoskeleton alter their morphology and function. Here, we demonstrated, for the first time, the structural changes in MS platelets that may be related to their hyperactivity. MS platelets were found to form large aggregates compared to control platelets. In contrast to the control, the images of overactivated, irregularly shaped MS platelets show changes in the cytoskeleton architecture, fragmented microtubule rings. Furthermore, MS platelets have long and numerous pseudopodia rich in actin filaments. We showed that MS platelets and megakaryocytes, overexpress β1-tubulin and β-actin mRNAs and proteins and have altered post-translational modification patterns. Moreover, we identified two previously undisclosed mutations in the gene encoding β1-tubulin in MS. We propose that the demonstrated structural changes of platelet cytoskeleton enhance their ability to adhere, aggregate, and degranulate fueling the risk of adverse cardiovascular events in MS.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:145 |
|---|---|
| Enthalten in: |
Journal of autoimmunity - 145(2024) vom: 22. März, Seite 103204 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Dziedzic, Angela [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
β-actin |
|---|
| Anmerkungen: |
Date Revised 23.03.2024 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1016/j.jaut.2024.103204 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370104730 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370104730 | ||
| 003 | DE-627 | ||
| 005 | 20240324235430.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240324s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jaut.2024.103204 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1345.xml |
| 035 | |a (DE-627)NLM370104730 | ||
| 035 | |a (NLM)38520895 | ||
| 035 | |a (PII)S0896-8411(24)00038-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Dziedzic, Angela |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quantitative and structural changes of blood platelet cytoskeleton proteins in multiple sclerosis (MS) |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 23.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Copyright © 2024. Published by Elsevier Ltd. | ||
| 520 | |a Epidemiological studies show that cardiovascular events related to platelet hyperactivity remain the leading causes of death among multiple sclerosis (MS) patients. Quantitative or structural changes of platelet cytoskeleton alter their morphology and function. Here, we demonstrated, for the first time, the structural changes in MS platelets that may be related to their hyperactivity. MS platelets were found to form large aggregates compared to control platelets. In contrast to the control, the images of overactivated, irregularly shaped MS platelets show changes in the cytoskeleton architecture, fragmented microtubule rings. Furthermore, MS platelets have long and numerous pseudopodia rich in actin filaments. We showed that MS platelets and megakaryocytes, overexpress β1-tubulin and β-actin mRNAs and proteins and have altered post-translational modification patterns. Moreover, we identified two previously undisclosed mutations in the gene encoding β1-tubulin in MS. We propose that the demonstrated structural changes of platelet cytoskeleton enhance their ability to adhere, aggregate, and degranulate fueling the risk of adverse cardiovascular events in MS | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Blood platelets | |
| 650 | 4 | |a Cytoskeleton proteins | |
| 650 | 4 | |a Megakaryocytes | |
| 650 | 4 | |a Multiple sclerosis | |
| 650 | 4 | |a Next generation sequencing | |
| 650 | 4 | |a Platelet overactivation | |
| 650 | 4 | |a Post-translational modifications | |
| 650 | 4 | |a β-actin | |
| 650 | 4 | |a β1-tubulin | |
| 700 | 1 | |a Michlewska, Sylwia |e verfasserin |4 aut | |
| 700 | 1 | |a Jóźwiak, Piotr |e verfasserin |4 aut | |
| 700 | 1 | |a Dębski, Janusz |e verfasserin |4 aut | |
| 700 | 1 | |a Karbownik, Michał Seweryn |e verfasserin |4 aut | |
| 700 | 1 | |a Łaczmański, Łukasz |e verfasserin |4 aut | |
| 700 | 1 | |a Kujawa, Dorota |e verfasserin |4 aut | |
| 700 | 1 | |a Glińska, Sława |e verfasserin |4 aut | |
| 700 | 1 | |a Miller, Elżbieta |e verfasserin |4 aut | |
| 700 | 1 | |a Niwald, Marta |e verfasserin |4 aut | |
| 700 | 1 | |a Kloc, Malgorzata |e verfasserin |4 aut | |
| 700 | 1 | |a Balcerzak, Łucja |e verfasserin |4 aut | |
| 700 | 1 | |a Saluk, Joanna |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of autoimmunity |d 1989 |g 145(2024) vom: 22. März, Seite 103204 |w (DE-627)NLM012637491 |x 1095-9157 |7 nnns |
| 773 | 1 | 8 | |g volume:145 |g year:2024 |g day:22 |g month:03 |g pages:103204 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jaut.2024.103204 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 145 |j 2024 |b 22 |c 03 |h 103204 | ||