A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature..
Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:169 |
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| Enthalten in: |
Archives of virology - 169(2024), 4 vom: 23. März, Seite 82 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Baek, Ji Hye [VerfasserIn] |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 08.04.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00705-024-06003-9 |
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| 245 | 1 | 2 | |a A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus |
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| 520 | |a © 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. | ||
| 520 | |a Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Vu, Ngoc Duong |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Min-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Jincun |e verfasserin |4 aut | |
| 700 | 1 | |a Le, Van Phan |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Joo Hyuk |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Jung-Eun |e verfasserin |4 aut | |
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