Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China
Copyright © 2024 Elsevier Inc. All rights reserved..
The occurrence of geminiviruses causes significant economic losses in many economically important crops. In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames. Phylogenetic and genome-wide pairwise identity analysis revealed that TLCCxV shared less than 91% identities with reported geminiviruses. A TLCCxV infectious clone was constructed and successfully infected Nicotiana benthamiana, N. tabacum, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants. Furthermore, expression of the V2, C1 and C4 proteins through a potato virus X vector caused severe chlorosis or necrosis symptom in N. benthamiana. Taken together, we identified a new geminivirus in tobacco plants, and found that V2, C1 and C4 contribute to symptom development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:594 |
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| Enthalten in: |
Virology - 594(2024) vom: 09. Apr., Seite 110061 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Yuan [VerfasserIn] |
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| Links: |
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| Themen: |
Begomovirus |
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| Anmerkungen: |
Date Completed 09.04.2024 Date Revised 09.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virol.2024.110061 |
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| 520 | |a The occurrence of geminiviruses causes significant economic losses in many economically important crops. In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames. Phylogenetic and genome-wide pairwise identity analysis revealed that TLCCxV shared less than 91% identities with reported geminiviruses. A TLCCxV infectious clone was constructed and successfully infected Nicotiana benthamiana, N. tabacum, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants. Furthermore, expression of the V2, C1 and C4 proteins through a potato virus X vector caused severe chlorosis or necrosis symptom in N. benthamiana. Taken together, we identified a new geminivirus in tobacco plants, and found that V2, C1 and C4 contribute to symptom development | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Pathogenicity | |
| 650 | 4 | |a Tomato leaf curl chuxiong virus | |
| 700 | 1 | |a Guo, Shiping |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Lianqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Fangfang |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Kaiqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhiping |e verfasserin |4 aut | |
| 700 | 1 | |a An, Mengnan |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Zihao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Fangfang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xueping |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yuanhua |e verfasserin |4 aut | |
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