Leukocyte Dysfunction and reduced CTLA-4 Expression are Associated with Perianal Crohn's Disease
© The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com..
Although perianal Crohn's disease (PCD) is highly associated with the exacerbated inflammation, the molecular basis and immunological signature that distinguish patients who present history of perianal lesions are still unclear. This paper aims to define immunological characteristics related to PCD. In this cross-sectional observational study, we enrolled 20 healthy controls and 39 CD patients. Blood samples were obtained for detection of plasma cytokines and LPS. PBMC were phenotyped by flow cytometry. Leukocytes were stimulated with LPS or anti-CD3/anti-CD28 antibodies. Our results show that CD patients had augmented plasma IL-6 and LPS. However, their PBMC was characterized by decreased IL-6 production, while patients with history of PCD produced higher IL-6, IL-8 and IFN-γ, along with decreased TNF. CD patients had augmented FoxP3 and CTLA-4 regulatory markers, though the PCD subjects presented a significant reduction in CTLA-4 expression. CTLA-4, as well as IL-6 and TNF responses were able to distinguish the PCD patients from those who did not present perianal complications. In conclusion, IL-6, TNF, and CTLA-4 exhibit a distinct expression pattern in CD patients with a history of PCD, regardless of disease activity. These findings clarify some mechanisms involved in the development of the perianal manifestations and may have a great impact in the disease management.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
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| Enthalten in: |
Clinical and experimental immunology - (2024) vom: 22. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Duarte-Silva, Murillo [VerfasserIn] |
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| Links: |
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| Themen: |
CTLA-4 |
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| Anmerkungen: |
Date Revised 22.03.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1093/cei/uxae027 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370066057 |
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| 520 | |a © The Author(s) 2024. Published by Oxford University Press on behalf of the British Society for Immunology. All rights reserved. For permissions, please e-mail: journals.permissionsoup.com. | ||
| 520 | |a Although perianal Crohn's disease (PCD) is highly associated with the exacerbated inflammation, the molecular basis and immunological signature that distinguish patients who present history of perianal lesions are still unclear. This paper aims to define immunological characteristics related to PCD. In this cross-sectional observational study, we enrolled 20 healthy controls and 39 CD patients. Blood samples were obtained for detection of plasma cytokines and LPS. PBMC were phenotyped by flow cytometry. Leukocytes were stimulated with LPS or anti-CD3/anti-CD28 antibodies. Our results show that CD patients had augmented plasma IL-6 and LPS. However, their PBMC was characterized by decreased IL-6 production, while patients with history of PCD produced higher IL-6, IL-8 and IFN-γ, along with decreased TNF. CD patients had augmented FoxP3 and CTLA-4 regulatory markers, though the PCD subjects presented a significant reduction in CTLA-4 expression. CTLA-4, as well as IL-6 and TNF responses were able to distinguish the PCD patients from those who did not present perianal complications. In conclusion, IL-6, TNF, and CTLA-4 exhibit a distinct expression pattern in CD patients with a history of PCD, regardless of disease activity. These findings clarify some mechanisms involved in the development of the perianal manifestations and may have a great impact in the disease management | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CTLA-4 | |
| 650 | 4 | |a Crohn’s disease | |
| 650 | 4 | |a Inflammatory bowel disease | |
| 650 | 4 | |a Interleukin-6 | |
| 650 | 4 | |a Perianal disease | |
| 700 | 1 | |a Parra, Rogério Serafim |e verfasserin |4 aut | |
| 700 | 1 | |a Feitosa, Marley Ribeiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nardini, Viviani |e verfasserin |4 aut | |
| 700 | 1 | |a Maruyama, Sandra Regina |e verfasserin |4 aut | |
| 700 | 1 | |a da Rocha, José Joaquim Ribeiro |e verfasserin |4 aut | |
| 700 | 1 | |a Feres, Omar |e verfasserin |4 aut | |
| 700 | 1 | |a de Barros Cardoso, Cristina Ribeiro |e verfasserin |4 aut | |
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