Detection of Bartonella schoenbuchensis (sub)species DNA in different louse fly species in Saxony, Germany : The proof of multiple PCR analysis necessity in case of ruminant-associated bartonellae determination
© 2024 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd..
BACKGROUND: Hippoboscid flies are bloodsucking arthropods that can transmit pathogenic microorganisms and are therefore potential vectors for pathogens such as Bartonella spp. These Gram-negative bacteria can cause mild-to-severe clinical signs in humans and animals; therefore, monitoring Bartonella spp. prevalence in louse fly populations appears to be a useful prerequisite for zoonotic risk assessment.
METHODS: Using convenience sampling, we collected 103 adult louse flies from four ked species (Lipoptena cervi, n = 22; Lipoptena fortisetosa, n = 61; Melophagus ovinus, n = 12; Hippobosca equina, n = 8) and the pupae of M. ovinus (n = 10) in the federal state of Saxony, Germany. All the samples were screened by polymerase chain reaction (PCR) for Bartonella spp. DNA, targeting the citrate synthase gene (gltA). Subsequently, PCRs targeting five more genes (16S, ftsZ, nuoG, ribC and rpoB) were performed for representatives of revealed gltA genotypes, and all the PCR products were sequenced to identify the Bartonella (sub)species accurately.
RESULTS AND CONCLUSIONS: The overall detection rates for Bartonella spp. were 100.0%, 59.1%, 24.6% and 75.0% in M. ovinus, L. cervi, L. fortisetosa and H. equina, respectively. All the identified bartonellae belong to the Bartonella schoenbuchensis complex. Our data support the proposed reclassification of the (sub)species status of this group, and thus we conclude that several genotypes of B. schoenbuchensis were detected, including Bartonella schoenbuchensis subsp. melophagi and Bartonella schoenbuchensis subsp. schoenbuchensis, both of which have previously validated zoonotic potential. The extensive PCR analysis revealed the necessity of multiple PCR approach for proper identification of the ruminant-associated bartonellae.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
|---|---|
| Enthalten in: |
Veterinary medicine and science - 10(2024), 3 vom: 22. März, Seite e1417 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Vogt, Isabelle [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 25.03.2024 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1002/vms3.1417 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM370064089 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM370064089 | ||
| 003 | DE-627 | ||
| 005 | 20240325235500.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240323s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/vms3.1417 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1347.xml |
| 035 | |a (DE-627)NLM370064089 | ||
| 035 | |a (NLM)38516829 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Vogt, Isabelle |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of Bartonella schoenbuchensis (sub)species DNA in different louse fly species in Saxony, Germany |b The proof of multiple PCR analysis necessity in case of ruminant-associated bartonellae determination |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.03.2024 | ||
| 500 | |a Date Revised 25.03.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2024 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: Hippoboscid flies are bloodsucking arthropods that can transmit pathogenic microorganisms and are therefore potential vectors for pathogens such as Bartonella spp. These Gram-negative bacteria can cause mild-to-severe clinical signs in humans and animals; therefore, monitoring Bartonella spp. prevalence in louse fly populations appears to be a useful prerequisite for zoonotic risk assessment | ||
| 520 | |a METHODS: Using convenience sampling, we collected 103 adult louse flies from four ked species (Lipoptena cervi, n = 22; Lipoptena fortisetosa, n = 61; Melophagus ovinus, n = 12; Hippobosca equina, n = 8) and the pupae of M. ovinus (n = 10) in the federal state of Saxony, Germany. All the samples were screened by polymerase chain reaction (PCR) for Bartonella spp. DNA, targeting the citrate synthase gene (gltA). Subsequently, PCRs targeting five more genes (16S, ftsZ, nuoG, ribC and rpoB) were performed for representatives of revealed gltA genotypes, and all the PCR products were sequenced to identify the Bartonella (sub)species accurately | ||
| 520 | |a RESULTS AND CONCLUSIONS: The overall detection rates for Bartonella spp. were 100.0%, 59.1%, 24.6% and 75.0% in M. ovinus, L. cervi, L. fortisetosa and H. equina, respectively. All the identified bartonellae belong to the Bartonella schoenbuchensis complex. Our data support the proposed reclassification of the (sub)species status of this group, and thus we conclude that several genotypes of B. schoenbuchensis were detected, including Bartonella schoenbuchensis subsp. melophagi and Bartonella schoenbuchensis subsp. schoenbuchensis, both of which have previously validated zoonotic potential. The extensive PCR analysis revealed the necessity of multiple PCR approach for proper identification of the ruminant-associated bartonellae | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bartonella schoenbuchensis | |
| 650 | 4 | |a Bartonella schoenbuchensis subsp. melophagi | |
| 650 | 4 | |a Hippobosca equina | |
| 650 | 4 | |a Hippoboscidae | |
| 650 | 4 | |a Lipoptena cervi | |
| 650 | 4 | |a Lipoptena fortisetosa | |
| 650 | 4 | |a Melophagus ovinus | |
| 650 | 4 | |a multiple polymerase chain reaction analysis | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 700 | 1 | |a Schröter, Stephanie |e verfasserin |4 aut | |
| 700 | 1 | |a Schreiter, Ruben |e verfasserin |4 aut | |
| 700 | 1 | |a Sprong, Hein |e verfasserin |4 aut | |
| 700 | 1 | |a Volfová, Karolina |e verfasserin |4 aut | |
| 700 | 1 | |a Jentzsch, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Freick, Markus |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Veterinary medicine and science |d 2015 |g 10(2024), 3 vom: 22. März, Seite e1417 |w (DE-627)NLM262254441 |x 2053-1095 |7 nnns |
| 773 | 1 | 8 | |g volume:10 |g year:2024 |g number:3 |g day:22 |g month:03 |g pages:e1417 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/vms3.1417 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 10 |j 2024 |e 3 |b 22 |c 03 |h e1417 | ||