Development of a Safe and Effective Bacillus thuringiensis-Based Nanobiopesticide for Controlling Tea Pests
Mg(OH)2 was used as the nanocarrier of the Bacillus thuringiensis (Bt) Cry1Ac protein, and the synthesized Cry1Ac-Mg(OH)2 composites were regular and uniform nanosheets. Nano-Mg(OH)2 could effectively improve the insecticidal effect of the Cry1Ac protein toward Ectropis obliqua. It could enhance the damage degree of the Cry1Ac protein to intestinal epithelial cells and microvilli, induce and enrich the production of reactive oxygen species (ROS) in the midgut, and enhance the degradation of the Cry1Ac protein into active fragments. Furthermore, an anti-rinsing assay showed that the Cry1Ac-Mg(OH)2 composites were bound to the notch structure of the tea leaf surface. The retention of the Cry1Ac protein increased by 11.45%, and sprayed nano-Mg(OH)2 was rapidly absorbed by different tissues of tea plants. Moreover, nano-Mg(OH)2 and composites did not significantly affect non-target organisms. These results show that nano-Mg(OH)2 can serve as a safe and effective biopesticide carrier, which provides a new approach for stable and efficient Bt preparation.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:72 |
---|---|
Enthalten in: |
Journal of agricultural and food chemistry - 72(2024), 14 vom: 10. Apr., Seite 7807-7817 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Pan, Xiaohong [VerfasserIn] |
---|
Links: |
---|
Themen: |
Bacterial Proteins |
---|
Anmerkungen: |
Date Completed 11.04.2024 Date Revised 11.04.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1021/acs.jafc.4c00833 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM370039688 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM370039688 | ||
003 | DE-627 | ||
005 | 20240411232516.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240323s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1021/acs.jafc.4c00833 |2 doi | |
028 | 5 | 2 | |a pubmed24n1372.xml |
035 | |a (DE-627)NLM370039688 | ||
035 | |a (NLM)38514390 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Pan, Xiaohong |e verfasserin |4 aut | |
245 | 1 | 0 | |a Development of a Safe and Effective Bacillus thuringiensis-Based Nanobiopesticide for Controlling Tea Pests |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 11.04.2024 | ||
500 | |a Date Revised 11.04.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Mg(OH)2 was used as the nanocarrier of the Bacillus thuringiensis (Bt) Cry1Ac protein, and the synthesized Cry1Ac-Mg(OH)2 composites were regular and uniform nanosheets. Nano-Mg(OH)2 could effectively improve the insecticidal effect of the Cry1Ac protein toward Ectropis obliqua. It could enhance the damage degree of the Cry1Ac protein to intestinal epithelial cells and microvilli, induce and enrich the production of reactive oxygen species (ROS) in the midgut, and enhance the degradation of the Cry1Ac protein into active fragments. Furthermore, an anti-rinsing assay showed that the Cry1Ac-Mg(OH)2 composites were bound to the notch structure of the tea leaf surface. The retention of the Cry1Ac protein increased by 11.45%, and sprayed nano-Mg(OH)2 was rapidly absorbed by different tissues of tea plants. Moreover, nano-Mg(OH)2 and composites did not significantly affect non-target organisms. These results show that nano-Mg(OH)2 can serve as a safe and effective biopesticide carrier, which provides a new approach for stable and efficient Bt preparation | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Cry protein | |
650 | 4 | |a bioactivity | |
650 | 4 | |a biosafety | |
650 | 4 | |a nano-Mg(OH)2 | |
650 | 4 | |a spatial distribution | |
650 | 7 | |a Bacterial Proteins |2 NLM | |
650 | 7 | |a Endotoxins |2 NLM | |
650 | 7 | |a Insecticides |2 NLM | |
650 | 7 | |a Hemolysin Proteins |2 NLM | |
650 | 7 | |a Tea |2 NLM | |
700 | 1 | |a Cao, Fang |e verfasserin |4 aut | |
700 | 1 | |a Guo, Xueping |e verfasserin |4 aut | |
700 | 1 | |a Wang, Yilin |e verfasserin |4 aut | |
700 | 1 | |a Cui, Ziqi |e verfasserin |4 aut | |
700 | 1 | |a Huang, Tianpei |e verfasserin |4 aut | |
700 | 1 | |a Hou, Youming |e verfasserin |4 aut | |
700 | 1 | |a Guan, Xiong |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Journal of agricultural and food chemistry |d 1967 |g 72(2024), 14 vom: 10. Apr., Seite 7807-7817 |w (DE-627)NLM000005665 |x 1520-5118 |7 nnns |
773 | 1 | 8 | |g volume:72 |g year:2024 |g number:14 |g day:10 |g month:04 |g pages:7807-7817 |
856 | 4 | 0 | |u http://dx.doi.org/10.1021/acs.jafc.4c00833 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 72 |j 2024 |e 14 |b 10 |c 04 |h 7807-7817 |