Intranasal Nose-to-Brain Drug Delivery via the Olfactory Region in Mice : Two In-Depth Protocols for Region-Specific Intranasal Application of Antibodies and for Expression Analysis of Fc Receptors via In Situ Hybridization in the Nasal Mucosa
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
A region-specific catheter-based intranasal administration method was successfully developed, established, and validated as reported previously. By using this method, drugs can be applicated specifically to the olfactory region. Thereby, intranasally administered drugs could be delivered via neuronal connections to the central nervous system. Here, we present a detailed protocol with a step-by-step procedure for nose-to-brain delivery via the olfactory mucosa.Fc receptors such as the neonatal Fc receptor (FcRn) and potentially Fcγ receptor IIb (FcγRIIb) are involved in the uptake and transport of antibodies via the olfactory nasal mucosa. To better characterize their expression levels and their role in CNS drug delivery via the nose, an in situ hybridization (ISH) protocol was adapted for nasal mucosa samples and described in abundant details.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2754 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2754(2024) vom: 21., Seite 387-410 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Herzog, Helena [VerfasserIn] |
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| Links: |
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| Themen: |
Antibodies |
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| Anmerkungen: |
Date Completed 25.03.2024 Date Revised 25.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3629-9_21 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM370022602 |
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| 520 | |a A region-specific catheter-based intranasal administration method was successfully developed, established, and validated as reported previously. By using this method, drugs can be applicated specifically to the olfactory region. Thereby, intranasally administered drugs could be delivered via neuronal connections to the central nervous system. Here, we present a detailed protocol with a step-by-step procedure for nose-to-brain delivery via the olfactory mucosa.Fc receptors such as the neonatal Fc receptor (FcRn) and potentially Fcγ receptor IIb (FcγRIIb) are involved in the uptake and transport of antibodies via the olfactory nasal mucosa. To better characterize their expression levels and their role in CNS drug delivery via the nose, an in situ hybridization (ISH) protocol was adapted for nasal mucosa samples and described in abundant details | ||
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| 650 | 4 | |a In situ hybridization | |
| 650 | 4 | |a Intranasal delivery | |
| 650 | 4 | |a Nose-to-brain drug delivery | |
| 650 | 4 | |a Olfactory mucosa | |
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| 650 | 7 | |a Antibodies |2 NLM | |
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| 700 | 1 | |a Flamm, Johannes |e verfasserin |4 aut | |
| 700 | 1 | |a Ladel, Simone |e verfasserin |4 aut | |
| 700 | 1 | |a Maigler, Frank |e verfasserin |4 aut | |
| 700 | 1 | |a Pitzer, Claudia |e verfasserin |4 aut | |
| 700 | 1 | |a Schindowski, Katharina |e verfasserin |4 aut | |
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