CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men
© 2024 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd..
Oligoasthenoteratospermia (OAT), characterized by abnormally low sperm count, poor sperm motility, and abnormally high number of deformed spermatozoa, is an important cause of male infertility. Its genetic basis in many affected individuals remains unknown. Here, we found that CCDC157 variants are associated with OAT. In two cohorts, a 21-bp (g.30768132_30768152del21) and/or 24-bp (g.30772543_30772566del24) deletion of CCDC157 were identified in five sporadic OAT patients, and 2 cases within one pedigree. In a mouse model, loss of Ccdc157 led to male sterility with OAT-like phenotypes. Electron microscopy revealed misstructured acrosome and abnormal head-tail coupling apparatus in the sperm of Ccdc157-null mice. Comparative transcriptome analysis showed that the Ccdc157 mutation alters the expressions of genes involved in cell migration/motility and Golgi components. Abnormal Golgi apparatus and decreased expressions of genes involved in acrosome formation and lipid metabolism were detected in Ccdc157-deprived mouse germ cells. Interestingly, we attempted to treat infertile patients and Ccdc157 mutant mice with a Chinese medicine, Huangjin Zanyu, which improved the fertility in one patient and most mice that carried the heterozygous mutation in CCDC157. Healthy offspring were produced. Our study reveals CCDC157 is essential for sperm maturation and may serve as a marker for diagnosis of OAT.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Journal of cellular and molecular medicine - 28(2024), 7 vom: 20. März, Seite e18215 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zheng, Huimei [VerfasserIn] |
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| Links: |
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| Themen: |
Acrosome |
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| Anmerkungen: |
Date Completed 22.03.2024 Date Revised 25.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1111/jcmm.18215 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Oligoasthenoteratospermia (OAT), characterized by abnormally low sperm count, poor sperm motility, and abnormally high number of deformed spermatozoa, is an important cause of male infertility. Its genetic basis in many affected individuals remains unknown. Here, we found that CCDC157 variants are associated with OAT. In two cohorts, a 21-bp (g.30768132_30768152del21) and/or 24-bp (g.30772543_30772566del24) deletion of CCDC157 were identified in five sporadic OAT patients, and 2 cases within one pedigree. In a mouse model, loss of Ccdc157 led to male sterility with OAT-like phenotypes. Electron microscopy revealed misstructured acrosome and abnormal head-tail coupling apparatus in the sperm of Ccdc157-null mice. Comparative transcriptome analysis showed that the Ccdc157 mutation alters the expressions of genes involved in cell migration/motility and Golgi components. Abnormal Golgi apparatus and decreased expressions of genes involved in acrosome formation and lipid metabolism were detected in Ccdc157-deprived mouse germ cells. Interestingly, we attempted to treat infertile patients and Ccdc157 mutant mice with a Chinese medicine, Huangjin Zanyu, which improved the fertility in one patient and most mice that carried the heterozygous mutation in CCDC157. Healthy offspring were produced. Our study reveals CCDC157 is essential for sperm maturation and may serve as a marker for diagnosis of OAT | ||
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| 700 | 1 | |a Gong, Chenjia |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jingping |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Jiaru |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Xinhan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Xinhai |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Huan |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Haoyue |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Fengbin |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Qinghua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jianteng |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Baolu |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiaohang |e verfasserin |4 aut | |
| 700 | 1 | |a Xi, Yongmei |e verfasserin |4 aut | |
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