Prevention of Metastasis by Suppression of Stemness Genes Using a Combination of microRNAs
We propose an original strategy for metastasis prevention using a combination of three microRNAs that blocks the dedifferentiation of cancer cells in a metastatic niche owing to the downregulation of stemness genes. Transcriptome microarray analysis was applied to identify the effects of a mixture of microRNAs on the pattern of differentially expressed genes in human breast cancer cell lines. Treatment of differentiated CD44- cancer cells with the microRNA mixture inhibited their ability to form mammospheres in vitro. The combination of these three microRNAs encapsulated into lipid nanoparticles prevented lung metastasis in a mouse model of spontaneous metastasis. The mixture of three microRNAs (miR-195-5p/miR-520a/miR-630) holds promise for the development of an antimetastatic therapeutic that blocks tumor cell dedifferentiation, which occurs at secondary tumor sites and determines the transition of micrometastases to macrometastases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
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| Enthalten in: |
Journal of medicinal chemistry - 67(2024), 7 vom: 11. Apr., Seite 5591-5602 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nevskaya, Kseniya V [VerfasserIn] |
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| Anmerkungen: |
Date Completed 12.04.2024 Date Revised 12.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.jmedchem.3c02199 |
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| funding: |
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NLM369973976 |
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| 520 | |a We propose an original strategy for metastasis prevention using a combination of three microRNAs that blocks the dedifferentiation of cancer cells in a metastatic niche owing to the downregulation of stemness genes. Transcriptome microarray analysis was applied to identify the effects of a mixture of microRNAs on the pattern of differentially expressed genes in human breast cancer cell lines. Treatment of differentiated CD44- cancer cells with the microRNA mixture inhibited their ability to form mammospheres in vitro. The combination of these three microRNAs encapsulated into lipid nanoparticles prevented lung metastasis in a mouse model of spontaneous metastasis. The mixture of three microRNAs (miR-195-5p/miR-520a/miR-630) holds promise for the development of an antimetastatic therapeutic that blocks tumor cell dedifferentiation, which occurs at secondary tumor sites and determines the transition of micrometastases to macrometastases | ||
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| 700 | 1 | |a Hmelevskaya, Ekaterina S |e verfasserin |4 aut | |
| 700 | 1 | |a Efimova, Lina V |e verfasserin |4 aut | |
| 700 | 1 | |a Ibragimova, Marina K |e verfasserin |4 aut | |
| 700 | 1 | |a Dolgasheva, Darya S |e verfasserin |4 aut | |
| 700 | 1 | |a Tsydenova, Irina A |e verfasserin |4 aut | |
| 700 | 1 | |a Ufandeev, Alexander A |e verfasserin |4 aut | |
| 700 | 1 | |a Buyko, Evgeny E |e verfasserin |4 aut | |
| 700 | 1 | |a Perina, Ekaterina A |e verfasserin |4 aut | |
| 700 | 1 | |a Gaptulbarova, Ksenia A |e verfasserin |4 aut | |
| 700 | 1 | |a Kravtsova, Ekaterina A |e verfasserin |4 aut | |
| 700 | 1 | |a Krivoshchekov, Sergei V |e verfasserin |4 aut | |
| 700 | 1 | |a Ivanov, Vladimir V |e verfasserin |4 aut | |
| 700 | 1 | |a Guriev, Artem M |e verfasserin |4 aut | |
| 700 | 1 | |a Udut, Elena V |e verfasserin |4 aut | |
| 700 | 1 | |a Litviakov, Nikolai V |e verfasserin |4 aut | |
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