Detection of SARS-CoV-2 virus in middle ear effusions and its association with otitis media with effusion
© 2024 Wiley Periodicals LLC..
A large-scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS-CoV-2, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS-CoV-2 virus was detected by RT-PCR targeting. The SARS-CoV-2 virus, angiotensin-converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID-19 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS-CoV-2 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33 ± 6.272 days after COVID-19 infection. The SARS-CoV-2 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019-2022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS-CoV-2 virus and the TMPRSS2 cofactors required for virus entry.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:96 |
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| Enthalten in: |
Journal of medical virology - 96(2024), 3 vom: 08. März, Seite e29545 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fu, Xiao [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.03.2024 Date Revised 21.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/jmv.29545 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a A large-scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS-CoV-2, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS-CoV-2 virus was detected by RT-PCR targeting. The SARS-CoV-2 virus, angiotensin-converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID-19 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS-CoV-2 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33 ± 6.272 days after COVID-19 infection. The SARS-CoV-2 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019-2022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS-CoV-2 virus and the TMPRSS2 cofactors required for virus entry | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a biostatistics & bioinformatics, RNA extraction | |
| 650 | 4 | |a biostatistics & bioinformatics, data processing | |
| 650 | 4 | |a coronavirus | |
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| 700 | 1 | |a Chen, Binjun |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Haojie |e verfasserin |4 aut | |
| 700 | 1 | |a Lyu, Jihan |e verfasserin |4 aut | |
| 700 | 1 | |a Shao, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Xiaoling |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Jianghong |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Juanmei |e verfasserin |4 aut | |
| 700 | 1 | |a Chi, Fanglu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Yibo |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Dongdong |e verfasserin |4 aut | |
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