Vasorin as an actor of bone turnover?
© 2024 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC..
Bone diseases are increasing with aging populations and it is important to identify clues to develop innovative treatments. Vasn, which encodes vasorin (Vasn), a transmembrane protein involved in the pathophysiology of several organs, is expressed during the development in intramembranous and endochondral ossification zones. Here, we studied the impact of Vasn deletion on the osteoblast and osteoclast dialog through a cell Coculture model. In addition, we explored the bone phenotype of Vasn KO mice, either constitutive or tamoxifen-inducible, or with an osteoclast-specific deletion. First, we show that both osteoblasts and osteoclasts express Vasn. Second, we report that, in both KO mouse models but not in osteoclast-targeted KO mice, Vasn deficiency was associated with an osteopenic bone phenotype, due to an imbalance in favor of osteoclastic resorption. Finally, through the Coculture experiments, we identify a dysregulation of the Wnt/β-catenin pathway together with an increase in RANKL release by osteoblasts, which led to an enhanced osteoclast activity. This study unravels a direct role of Vasn in bone turnover, introducing a new biomarker or potential therapeutic target for bone pathologies.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2024 |
|---|---|
| Enthalten in: |
Journal of cellular physiology - (2024) vom: 19. März |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Andrique, Caroline [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Revised 20.03.2024 published: Print-Electronic Citation Status Publisher |
|---|
| doi: |
10.1002/jcp.31257 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM369940989 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM369940989 | ||
| 003 | DE-627 | ||
| 005 | 20240320235405.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 240320s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/jcp.31257 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1337.xml |
| 035 | |a (DE-627)NLM369940989 | ||
| 035 | |a (NLM)38504496 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Andrique, Caroline |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Vasorin as an actor of bone turnover? |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 20.03.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a © 2024 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC. | ||
| 520 | |a Bone diseases are increasing with aging populations and it is important to identify clues to develop innovative treatments. Vasn, which encodes vasorin (Vasn), a transmembrane protein involved in the pathophysiology of several organs, is expressed during the development in intramembranous and endochondral ossification zones. Here, we studied the impact of Vasn deletion on the osteoblast and osteoclast dialog through a cell Coculture model. In addition, we explored the bone phenotype of Vasn KO mice, either constitutive or tamoxifen-inducible, or with an osteoclast-specific deletion. First, we show that both osteoblasts and osteoclasts express Vasn. Second, we report that, in both KO mouse models but not in osteoclast-targeted KO mice, Vasn deficiency was associated with an osteopenic bone phenotype, due to an imbalance in favor of osteoclastic resorption. Finally, through the Coculture experiments, we identify a dysregulation of the Wnt/β-catenin pathway together with an increase in RANKL release by osteoblasts, which led to an enhanced osteoclast activity. This study unravels a direct role of Vasn in bone turnover, introducing a new biomarker or potential therapeutic target for bone pathologies | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a bone | |
| 650 | 4 | |a osteoblast | |
| 650 | 4 | |a osteoclast | |
| 650 | 4 | |a physiology | |
| 700 | 1 | |a Bonnet, Anne Laure |e verfasserin |4 aut | |
| 700 | 1 | |a Dang, Julien |e verfasserin |4 aut | |
| 700 | 1 | |a Lesieur, Julie |e verfasserin |4 aut | |
| 700 | 1 | |a Krautzberger, A Michaela |e verfasserin |4 aut | |
| 700 | 1 | |a Baroukh, Brigitte |e verfasserin |4 aut | |
| 700 | 1 | |a Torrens, Coralie |e verfasserin |4 aut | |
| 700 | 1 | |a Sadoine, Jeremy |e verfasserin |4 aut | |
| 700 | 1 | |a Schmitt, Alain |e verfasserin |4 aut | |
| 700 | 1 | |a Rochefort, Gael Y |e verfasserin |4 aut | |
| 700 | 1 | |a Bardet, Claire |e verfasserin |4 aut | |
| 700 | 1 | |a Six, Isabelle |e verfasserin |4 aut | |
| 700 | 1 | |a Houillier, Pascal |e verfasserin |4 aut | |
| 700 | 1 | |a Tharaux, Pierre Louis |e verfasserin |4 aut | |
| 700 | 1 | |a Schrewe, Heinrich |e verfasserin |4 aut | |
| 700 | 1 | |a Gaucher, Celine |e verfasserin |4 aut | |
| 700 | 1 | |a Chaussain, Catherine |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of cellular physiology |d 1965 |g (2024) vom: 19. März |w (DE-627)NLM000005304 |x 1097-4652 |7 nnns |
| 773 | 1 | 8 | |g year:2024 |g day:19 |g month:03 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/jcp.31257 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 19 |c 03 | ||