DT-13 attenuates inflammation by inhibiting NLRP3-inflammasome related genes in RAW264.7 macrophages
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved..
Plant derived saponins or other glycosides are widely used for their anti-inflammatory, antioxidant, and anti-viral properties in therapeutic medicine. In this study, we focus on understanding the function of the less known steroidal saponin from the roots of Liriope muscari L. H. Bailey - saponin C (also known as DT-13) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison to the well-known saponin ginsenoside Rk1 and anti-inflammatory drug dexamethasone. We proved that DT-13 reduces LPS-induced inflammation by inhibiting nitric oxide (NO) production, interleukin-6 (IL-6) release, cycloxygenase-2 (COX-2), tumour necrosis factor-alpha (TNF-α) gene expression, and nuclear factor kappa-B (NFκB) translocation into the nucleus. It also inhibits the inflammasome component NOD-like receptor family pyrin domain containing protein 3 (NLRP3) regulating the inflammasome activation. This was supported by the significant inhibition of caspase-1 and interleukin-1 beta (IL-1β) expression and release. This study demonstrates the anti-inflammatory effect of saponins on LPS-stimulated macrophages. For the first time, an in vitro study shows the attenuating effect of DT-13 on NLRP3-inflammasome activation. In comparison to the existing anti-inflammatory drug, dexamethasone, and triterpenoid saponin Rk1, DT-13 more efficiently inhibits inflammation in the applied cell culture model. Therefore, DT-13 may serve as a lead compound for the development of new more effective anti-inflammatory drugs with minimised side effects.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
Zur Gesamtaufnahme - volume:708 |
---|---|
Enthalten in: |
Biochemical and biophysical research communications - 708(2024) vom: 14. Apr., Seite 149763 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Raina, Shikha [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 08.04.2024 Date Revised 08.04.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.bbrc.2024.149763 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM369927788 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM369927788 | ||
003 | DE-627 | ||
005 | 20240408232744.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240320s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.bbrc.2024.149763 |2 doi | |
028 | 5 | 2 | |a pubmed24n1369.xml |
035 | |a (DE-627)NLM369927788 | ||
035 | |a (NLM)38503169 | ||
035 | |a (PII)S0006-291X(24)00299-7 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Raina, Shikha |e verfasserin |4 aut | |
245 | 1 | 0 | |a DT-13 attenuates inflammation by inhibiting NLRP3-inflammasome related genes in RAW264.7 macrophages |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 08.04.2024 | ||
500 | |a Date Revised 08.04.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved. | ||
520 | |a Plant derived saponins or other glycosides are widely used for their anti-inflammatory, antioxidant, and anti-viral properties in therapeutic medicine. In this study, we focus on understanding the function of the less known steroidal saponin from the roots of Liriope muscari L. H. Bailey - saponin C (also known as DT-13) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages in comparison to the well-known saponin ginsenoside Rk1 and anti-inflammatory drug dexamethasone. We proved that DT-13 reduces LPS-induced inflammation by inhibiting nitric oxide (NO) production, interleukin-6 (IL-6) release, cycloxygenase-2 (COX-2), tumour necrosis factor-alpha (TNF-α) gene expression, and nuclear factor kappa-B (NFκB) translocation into the nucleus. It also inhibits the inflammasome component NOD-like receptor family pyrin domain containing protein 3 (NLRP3) regulating the inflammasome activation. This was supported by the significant inhibition of caspase-1 and interleukin-1 beta (IL-1β) expression and release. This study demonstrates the anti-inflammatory effect of saponins on LPS-stimulated macrophages. For the first time, an in vitro study shows the attenuating effect of DT-13 on NLRP3-inflammasome activation. In comparison to the existing anti-inflammatory drug, dexamethasone, and triterpenoid saponin Rk1, DT-13 more efficiently inhibits inflammation in the applied cell culture model. Therefore, DT-13 may serve as a lead compound for the development of new more effective anti-inflammatory drugs with minimised side effects | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Anti-inflammatory effects | |
650 | 4 | |a NFκB pathway | |
650 | 4 | |a NLRP3 inflammasome | |
650 | 4 | |a Steroidal saponin | |
650 | 7 | |a Inflammasomes |2 NLM | |
650 | 7 | |a NLR Family, Pyrin Domain-Containing 3 Protein |2 NLM | |
650 | 7 | |a Lipopolysaccharides |2 NLM | |
650 | 7 | |a Anti-Inflammatory Agents |2 NLM | |
650 | 7 | |a NF-kappa B |2 NLM | |
650 | 7 | |a Dexamethasone |2 NLM | |
650 | 7 | |a 7S5I7G3JQL |2 NLM | |
700 | 1 | |a Hübner, Emely |e verfasserin |4 aut | |
700 | 1 | |a Samuel, Esther |e verfasserin |4 aut | |
700 | 1 | |a Nagel, Gregor |e verfasserin |4 aut | |
700 | 1 | |a Fuchs, Hendrik |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d 1960 |g 708(2024) vom: 14. Apr., Seite 149763 |w (DE-627)NLM000000035 |x 1090-2104 |7 nnns |
773 | 1 | 8 | |g volume:708 |g year:2024 |g day:14 |g month:04 |g pages:149763 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.bbrc.2024.149763 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 708 |j 2024 |b 14 |c 04 |h 149763 |