A highly sensitive Lock-Cas12a biosensor for detection and imaging of miRNA-21 in breast cancer cells
Copyright © 2024 Elsevier B.V. All rights reserved..
The expression levels of microRNA (miRNA) vary significantly in correlation with the occurrence and progression of cancer, making them valuable biomarkers for cancer diagnosis. However, their quantitative detection faces challenges due to the high sequence homology, low abundance and small size. In this work, we established a strand displacement amplification (SDA) approach based on miRNA-triggered structural "Lock" nucleic acid ("Lock" DNA), coupled with the CRISPR/Cas12a system, for detecting miRNA-21 in breast cancer cells. The "Lock" DNA freed the CRISPR-derived RNA (crRNA) from the dependence on the target sequence and greatly facilitated the extended detection of different miRNAs. Moreover, the CRISPR/Cas12a system provided excellent amplification ability and specificity. The designed biosensor achieved high sensitivity detection of miRNA-21 with a limit of detection (LOD) of 28.8 aM. In particular, the biosensor could distinguish breast cancer cells from other cancer cells through intracellular imaging. With its straightforward sequence design and ease of use, the Lock-Cas12a biosensor offers significant advantages for cell imaging and early clinical diagnosis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:273 |
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| Enthalten in: |
Talanta - 273(2024) vom: 01. Apr., Seite 125938 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Peng, Jiawei [VerfasserIn] |
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| Links: |
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| Themen: |
Bioanalysis |
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| Anmerkungen: |
Date Completed 09.04.2024 Date Revised 09.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.talanta.2024.125938 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
| 520 | |a The expression levels of microRNA (miRNA) vary significantly in correlation with the occurrence and progression of cancer, making them valuable biomarkers for cancer diagnosis. However, their quantitative detection faces challenges due to the high sequence homology, low abundance and small size. In this work, we established a strand displacement amplification (SDA) approach based on miRNA-triggered structural "Lock" nucleic acid ("Lock" DNA), coupled with the CRISPR/Cas12a system, for detecting miRNA-21 in breast cancer cells. The "Lock" DNA freed the CRISPR-derived RNA (crRNA) from the dependence on the target sequence and greatly facilitated the extended detection of different miRNAs. Moreover, the CRISPR/Cas12a system provided excellent amplification ability and specificity. The designed biosensor achieved high sensitivity detection of miRNA-21 with a limit of detection (LOD) of 28.8 aM. In particular, the biosensor could distinguish breast cancer cells from other cancer cells through intracellular imaging. With its straightforward sequence design and ease of use, the Lock-Cas12a biosensor offers significant advantages for cell imaging and early clinical diagnosis | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Biosensors | |
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| 650 | 4 | |a Strand displacement amplification | |
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| 700 | 1 | |a Liu, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Liwen |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Ziyue |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jiaxin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xuexia |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yifei |e verfasserin |4 aut | |
| 700 | 1 | |a Hao, Xian |e verfasserin |4 aut | |
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