Culture and Maintenance of Immune Cells to Model Innate Immune Status at the Feto-maternal Interface
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2781 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2781(2024) vom: 19., Seite 119-130 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lintao, Ryan C V [VerfasserIn] |
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| Links: |
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| Themen: |
Cell culture |
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| Anmerkungen: |
Date Completed 21.03.2024 Date Revised 21.03.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-3746-3_11 |
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| 520 | |a The inflammatory process leading to human labor is mostly facilitated by immune cells, which can be studied by isolating and characterizing primary immune cells from the feto-maternal interface. However, difficulty and inconsistency in sampling approaches of immune cells and short lifespan in vitro prevent their usage in mechanistic studies to understand the maternal-fetal immunobiology. To address these limitations, existing cell line models can be differentiated into immune-like cells for use in reproductive biology experiments. In this chapter, we discussed cell culture methods of maintaining and differentiating HL-60, THP-1, and NK-92 cells to obtain neutrophil-like, macrophage-like, and decidual natural killer-like cells, respectively, which can then be used together with intrauterine cells to elucidate and investigate immune mechanisms that contribute to parturition | ||
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| 700 | 1 | |a Menon, Ramkumar |e verfasserin |4 aut | |
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